Evaluation of cyclosporine-induced nephrotoxicity by immunoperoxidase analysis of renal allograft biopsies

P. C. Kolbeck, J. A. Wolfe, J. Burchette, F. Sanfilippo

Research output: Contribution to journalArticlepeer-review


Renal allograft biopsies are frequently performed in order to distinguish rejection from cyclosporine nephrotoxicity (CsA-NT). Histopathologic evaluation alone or in conjunction with clinical information, however, has not been shown to be effective in diagnosing CsA-NT. The critical differences in treatment required for CsA-NT (decreased CsA dosage) v rejection (increased immunosuppression) make the accurate identification of CsA-NT an important clinical problem in renal transplantation. Recently, we have reported an association between cellular rejection and interstitial infiltrates of predominantly Leu 2 (CD8) positive (T cytotoxic-suppressor, T(c-s)) cells, which may be helpful in distinguishing rejection from CsA-NT. von Willebrand and Hayry have reported that deposits of CsA could be identified by CsA antibody labeling of tubular epithelial cells (fine-needle aspiration cytology specimens) from cases of presumed CsA-NT. In this study, we have performed immunoperoxidase labeling with polyclonal antibody to CsA in a series of renal allograft biopsies obtained from patients treated with CsA in order to characterize the specificity and pattern of CsA deposition within renal allograft tissue.

Original languageEnglish (US)
Pages (from-to)1781-1784
Number of pages4
JournalTransplantation Proceedings
Issue number1 II
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Transplantation


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