Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells

Hua Ye; Zhidao Xia; David Ferguson; James T. Triffitt; Zhanfeng Cui

Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells

Hua Ye
, Zhidao Xia
, David Ferguson
, James T. Triffitt
, Zhanfeng Cui

Research output: Contribution to conference › Paper › peer-review

Abstract

The biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic (HBMF) stem cells was evaluated. The cells were examined by light and fluorescence microscopy after culture for up to 21 days in vitro and then implanted subcutaneously into severe combined immunodeficient/non-obese diabetic mice. The results show that after 21 days in vitro, EGFP-cells could readily be detected by their green fluorescence under incident fluorescence microscopy. It was found that the use of RGFP-labelled HBMF cells is an effective tool to evaluate the interactions between the cells and biomaterials both in vivo and vitro.

Original language	English (US)
Pages	130
Number of pages	1
State	Published - 2002
Externally published	Yes
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States Duration: Oct 13 2002 → Oct 16 2002

Conference

Conference	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta, GA
Period	10/13/02 → 10/16/02

ASJC Scopus subject areas

General Engineering

Cite this

Ye, H., Xia, Z., Ferguson, D., Triffitt, J. T., & Cui, Z. (2002). Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells. 130. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells. / Ye, Hua; Xia, Zhidao; Ferguson, David et al.
2002. 130 Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Research output: Contribution to conference › Paper › peer-review

Ye, H, Xia, Z, Ferguson, D, Triffitt, JT & Cui, Z 2002, 'Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells', Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States, 10/13/02 - 10/16/02 pp. 130.

Ye H, Xia Z, Ferguson D, Triffitt JT, Cui Z. Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells. 2002. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Ye, Hua ; Xia, Zhidao ; Ferguson, David et al. / Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.1 p.

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title = "Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells",

abstract = "The biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic (HBMF) stem cells was evaluated. The cells were examined by light and fluorescence microscopy after culture for up to 21 days in vitro and then implanted subcutaneously into severe combined immunodeficient/non-obese diabetic mice. The results show that after 21 days in vitro, EGFP-cells could readily be detected by their green fluorescence under incident fluorescence microscopy. It was found that the use of RGFP-labelled HBMF cells is an effective tool to evaluate the interactions between the cells and biomaterials both in vivo and vitro.",

author = "Hua Ye and Zhidao Xia and David Ferguson and Triffitt, \{James T.\} and Zhanfeng Cui",

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AU - Xia, Zhidao

AU - Ferguson, David

AU - Triffitt, James T.

AU - Cui, Zhanfeng

PY - 2002

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AB - The biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic (HBMF) stem cells was evaluated. The cells were examined by light and fluorescence microscopy after culture for up to 21 days in vitro and then implanted subcutaneously into severe combined immunodeficient/non-obese diabetic mice. The results show that after 21 days in vitro, EGFP-cells could readily be detected by their green fluorescence under incident fluorescence microscopy. It was found that the use of RGFP-labelled HBMF cells is an effective tool to evaluate the interactions between the cells and biomaterials both in vivo and vitro.

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M3 - Paper

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Y2 - 13 October 2002 through 16 October 2002

ER -

Evaluation of biocompatibility of scaffolds for orthopaedic tissue engineering using enhanced green fluorescent protein (EGFP)-labeled human bone marrow fibroblastic stem cells

Abstract

Conference

ASJC Scopus subject areas

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