Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection

Rosario Vivek; Syed Fazil Ahamed; Shalini Kotabagi; Anmol Chandele; Ira Khanna; Navin Khanna; Kaustuv Nayak; Mary Dias; Murali Krishna Kaja; Anita Shet

doi:10.1016/j.diagmicrobio.2016.09.020

Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection

Rosario Vivek, Syed Fazil Ahamed, Shalini Kotabagi, Anmol Chandele, Ira Khanna, Navin Khanna, Kaustuv Nayak, Mary Dias, Murali Krishna Kaja, Anita Shet

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

Original language	English (US)
Pages (from-to)	229-234
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	87
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.09.020
State	Published - Mar 1 2017

Keywords

Dengue
Diagnostics
Evaluation
NS1 protein
Rapid diagnostic test

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2016.09.020

Cite this

@article{a329984aac5d441fad3379672f90c79a,

title = "Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection",

abstract = "The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.",

keywords = "Dengue, Diagnostics, Evaluation, NS1 protein, Rapid diagnostic test",

author = "Rosario Vivek and Ahamed, {Syed Fazil} and Shalini Kotabagi and Anmol Chandele and Ira Khanna and Navin Khanna and Kaustuv Nayak and Mary Dias and Kaja, {Murali Krishna} and Anita Shet",

note = "Publisher Copyright: {\textcopyright} 2016",

year = "2017",

month = mar,

day = "1",

doi = "10.1016/j.diagmicrobio.2016.09.020",

language = "English (US)",

volume = "87",

pages = "229--234",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection

AU - Vivek, Rosario

AU - Ahamed, Syed Fazil

AU - Kotabagi, Shalini

AU - Chandele, Anmol

AU - Khanna, Ira

AU - Khanna, Navin

AU - Nayak, Kaustuv

AU - Dias, Mary

AU - Kaja, Murali Krishna

AU - Shet, Anita

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

AB - The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

KW - Dengue

KW - Diagnostics

KW - Evaluation

KW - NS1 protein

KW - Rapid diagnostic test

UR - http://www.scopus.com/inward/record.url?scp=85007557086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007557086&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2016.09.020

DO - 10.1016/j.diagmicrobio.2016.09.020

M3 - Article

C2 - 27955870

AN - SCOPUS:85007557086

SN - 0732-8893

VL - 87

SP - 229

EP - 234

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this