@article{61aec3402b7f4077856c3ddf66c3bfa2,
title = "Evaluation of a multiplex PCR assay to rapidly detect Enterobacteriaceae with a broad range of β-lactamases directly from perianal swabs",
abstract = "We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.",
author = "Chavda, {Kalyan D.} and Satlin, {Michael J.} and Liang Chen and Claudia Manca and Jenkins, {Stephen G.} and Walsh, {Thomas J.} and Kreiswirth, {Barry N.}",
note = "Funding Information: This study was supported by a grant from the New York Community Trust to B.N.K. and grants R01AI090155 to B.N.K., R21AI117338 to L.C., and K23AI114994 to M.J.S. from National Institute of Allergy and Infectious Diseases. This work, including the efforts of Barry N. Kreiswirth, was funded by HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (R01AI090155). This work, including the efforts of Liang Chen, was funded by HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (R21AI117338). This work, including the efforts of Michael J. Satlin, was funded by HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (K23AI114994). Publisher Copyright: Copyright {\textcopyright} 2016, American Society for Microbiology. All Rights Reserved.",
year = "2016",
month = nov,
day = "1",
doi = "10.1128/AAC.01458-16",
language = "English (US)",
volume = "60",
pages = "6957--6961",
journal = "Antimicrobial agents and chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "11",
}