Estradiol reverses the limiting effects of clomiphene citrate on early embryonic development in the in vitro perfused rabbit ovary

Y. Yoshimura; T. Adachi; Y. Hosoi; R. Santulli; S. J. Atlas; E. E. Wallach

doi:10.1016/s0015-0282(16)59604-6

Estradiol reverses the limiting effects of clomiphene citrate on early embryonic development in the in vitro perfused rabbit ovary

Y. Yoshimura, T. Adachi, Y. Hosoi, R. Santulli, S. J. Atlas, E. E. Wallach

School of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This study examined whether the addition of estradiol (E₂) to the perfused rabbit ovary would reverse the deleterious effects of clomiphene citrate (CC) on early embryonic development. Ovaries were perfused with CC (10^-5 M) or CC + E₂ (1 to 1000 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of each ovary. In vitro ovulated ova in cumulus were retrieved and inseminated in vitro. E₂ significantly increased the percentage of ovulated ova achieving (1) the 2-cell stage at 36 hours, (2) the morula stage by 84 hours, and (3) the blastocyst stage at 132 hours. The percentage of inseminated ova showing evidence of degeneration was reduced in ovaries treated with E₂. These data suggest that CC may exert an antiestrogenic effect on the intrafollicular oocyte, which interferes with postfertilization development.

Original language	English (US)
Pages (from-to)	1030-1035
Number of pages	6
Journal	Fertility and sterility
Volume	48
Issue number	6
DOIs	https://doi.org/10.1016/s0015-0282(16)59604-6
State	Published - 1987

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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title = "Estradiol reverses the limiting effects of clomiphene citrate on early embryonic development in the in vitro perfused rabbit ovary",

abstract = "This study examined whether the addition of estradiol (E2) to the perfused rabbit ovary would reverse the deleterious effects of clomiphene citrate (CC) on early embryonic development. Ovaries were perfused with CC (10-5 M) or CC + E2 (1 to 1000 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of each ovary. In vitro ovulated ova in cumulus were retrieved and inseminated in vitro. E2 significantly increased the percentage of ovulated ova achieving (1) the 2-cell stage at 36 hours, (2) the morula stage by 84 hours, and (3) the blastocyst stage at 132 hours. The percentage of inseminated ova showing evidence of degeneration was reduced in ovaries treated with E2. These data suggest that CC may exert an antiestrogenic effect on the intrafollicular oocyte, which interferes with postfertilization development.",

author = "Y. Yoshimura and T. Adachi and Y. Hosoi and R. Santulli and Atlas, {S. J.} and Wallach, {E. E.}",

note = "Funding Information: Received May 18, 1987; revised and accepted July 21, 1987. * Supported by the National Institutes of Health grant HD-19430, the Rockefeller Foundation, the Connelly Foundation, and the Mitchell and Lillian Duberstein Foundation. t Presented in part at the forty-second annual meeting of The American Fertility Society and the eighteenth annual meeting of The Canadian Fertility and Andrology Society, September 27 to October 2, 1986, Toronto, Ontario, Canada. :j: Reprint requests: Edward E. Wallach, M.D., Department of Gynecology and Obstetrics, The Johns Hopkins Hospital, 601 North Wolfe Street, Houck Building-Room 264, Baltimore, Maryland 21205.",

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AU - Yoshimura, Y.

AU - Adachi, T.

AU - Hosoi, Y.

AU - Santulli, R.

AU - Atlas, S. J.

AU - Wallach, E. E.

N1 - Funding Information: Received May 18, 1987; revised and accepted July 21, 1987. * Supported by the National Institutes of Health grant HD-19430, the Rockefeller Foundation, the Connelly Foundation, and the Mitchell and Lillian Duberstein Foundation. t Presented in part at the forty-second annual meeting of The American Fertility Society and the eighteenth annual meeting of The Canadian Fertility and Andrology Society, September 27 to October 2, 1986, Toronto, Ontario, Canada. :j: Reprint requests: Edward E. Wallach, M.D., Department of Gynecology and Obstetrics, The Johns Hopkins Hospital, 601 North Wolfe Street, Houck Building-Room 264, Baltimore, Maryland 21205.

PY - 1987

Y1 - 1987

N2 - This study examined whether the addition of estradiol (E2) to the perfused rabbit ovary would reverse the deleterious effects of clomiphene citrate (CC) on early embryonic development. Ovaries were perfused with CC (10-5 M) or CC + E2 (1 to 1000 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of each ovary. In vitro ovulated ova in cumulus were retrieved and inseminated in vitro. E2 significantly increased the percentage of ovulated ova achieving (1) the 2-cell stage at 36 hours, (2) the morula stage by 84 hours, and (3) the blastocyst stage at 132 hours. The percentage of inseminated ova showing evidence of degeneration was reduced in ovaries treated with E2. These data suggest that CC may exert an antiestrogenic effect on the intrafollicular oocyte, which interferes with postfertilization development.

AB - This study examined whether the addition of estradiol (E2) to the perfused rabbit ovary would reverse the deleterious effects of clomiphene citrate (CC) on early embryonic development. Ovaries were perfused with CC (10-5 M) or CC + E2 (1 to 1000 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of each ovary. In vitro ovulated ova in cumulus were retrieved and inseminated in vitro. E2 significantly increased the percentage of ovulated ova achieving (1) the 2-cell stage at 36 hours, (2) the morula stage by 84 hours, and (3) the blastocyst stage at 132 hours. The percentage of inseminated ova showing evidence of degeneration was reduced in ovaries treated with E2. These data suggest that CC may exert an antiestrogenic effect on the intrafollicular oocyte, which interferes with postfertilization development.

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Estradiol reverses the limiting effects of clomiphene citrate on early embryonic development in the in vitro perfused rabbit ovary

Abstract

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