@article{3da28cc69aa947d9a98a4236920f65f3,
title = "Essential function and targets of BMP signaling during midbrain neural crest delamination",
abstract = "BMP signaling plays iterative roles during vertebrate neural crest development from induction through craniofacial morphogenesis. However, far less is known about the role of BMP activity in cranial neural crest epithelial-to-mesenchymal transition and delamination. By measuring canonical BMP signaling activity as a function of time from specification through early migration of avian midbrain neural crest cells, we found elevated BMP signaling during delamination stages. Moreover, inhibition of canonical BMP activity via a dominant negative mutant Type I BMP receptor showed that BMP signaling is required for neural crest migration from the midbrain, independent from an effect on EMT and delamination. Transcriptome profiling on control compared to BMP-inhibited cranial neural crest cells identified novel BMP targets during neural crest delamination and early migration including targets of the Notch pathway that are upregulated following BMP inhibition. These results suggest potential crosstalk between the BMP and Notch pathways in early migrating cranial neural crest and provide novel insight into mechanisms regulated by BMP signaling during early craniofacial development.",
keywords = "BMP signaling, Delamination, Epithelial-to-mesenchymal transition, Migration, Neural crest, RNA Seq",
author = "Piacentino, {Michael L.} and Hutchins, {Erica J.} and Bronner, {Marianne E.}",
note = "Funding Information: We would like to thank Megan Martik and Shashank Gandhi for valuable discussion on experiment design and analysis, and Gabriel da Silva Pescador for technical support. We thank Elisa Mart{\'i} for sharing reagents, Patrick Cannon and Rochelle Diamond at the Caltech Flow Cytometry Cell Sorting Facility for cell sorting, Igor Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for library sequencing, and the Beckman Institute Biological Imaging Facility for microscopy support. Funding for this work comes from the National Institutes of Health grants K99DE029240 to M.L.P., K99DE028592 to E.J.H, R01DE027538 and R01DE027568 to M.E.B. Funding Information: We would like to thank Megan Martik and Shashank Gandhi for valuable discussion on experiment design and analysis, and Gabriel da Silva Pescador for technical support. We thank Elisa Mart? for sharing reagents, Patrick Cannon and Rochelle Diamond at the Caltech Flow Cytometry Cell Sorting Facility for cell sorting, Igor Antoshechkin of the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for library sequencing, and the Beckman Institute Biological Imaging Facility for microscopy support. Funding for this work comes from the National Institutes of Health grants K99DE029240 to M.L.P. K99DE028592 to E.J.H, R01DE027538 and R01DE027568 to M.E.B. Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = sep,
doi = "10.1016/j.ydbio.2021.06.003",
language = "English (US)",
volume = "477",
pages = "251--261",
journal = "Developmental biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
}