TY - JOUR
T1 - Erythropoietin
T2 - isolation by affinity chromatography with lectin-agarose derivatives
AU - Spivak, J. L.
AU - Small, D.
AU - Hollenberg, M. D.
PY - 1977
Y1 - 1977
N2 - Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. On the basis of previous estimates of the sugar content of the hormone, six lectins (wheat germ agglutinin, phytohemagglutinin, Ricinus communis 120, soybean agglutinin, concanavalin A, and limulin) were chosen for study. Only wheat germ agglutinin-agarose and phytohemagglutinin-agarose derivatives had significant affinity for erythropoietin. By use of wheat germ agglutinin-agarose columns, erythropoietin could be separated from over 95% of the initial starting protein, resulting in an 8- to 100-fold purification and a recovery of at least 40% depending on the source of the hormone. Affinity chromatography with agarose-bound lectins provides a simple rapid method for isolating erythropoietin from crude preparations of the hormone.
AB - Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. On the basis of previous estimates of the sugar content of the hormone, six lectins (wheat germ agglutinin, phytohemagglutinin, Ricinus communis 120, soybean agglutinin, concanavalin A, and limulin) were chosen for study. Only wheat germ agglutinin-agarose and phytohemagglutinin-agarose derivatives had significant affinity for erythropoietin. By use of wheat germ agglutinin-agarose columns, erythropoietin could be separated from over 95% of the initial starting protein, resulting in an 8- to 100-fold purification and a recovery of at least 40% depending on the source of the hormone. Affinity chromatography with agarose-bound lectins provides a simple rapid method for isolating erythropoietin from crude preparations of the hormone.
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U2 - 10.1073/pnas.74.10.4633
DO - 10.1073/pnas.74.10.4633
M3 - Article
C2 - 270704
AN - SCOPUS:0017712715
SN - 0027-8424
VL - 74
SP - 4633
EP - 4635
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -