ERAAP and tapasin independently edit the amino and carboxyl termini of MHC class I peptides

Takayuki Kanaseki, Kristin Camfield Lind, Hernando Escobar, Niranjana Nagarajan, Eduardo Reyes-Vargas, Brant Rudd, Alan L. Rockwood, Luc Van Kaer, Noriyuki Sato, Julio C. Delgado, Nilabh Shastri

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Effective CD8+ T cell responses depend on presentation of a stable peptide repertoire by MHC class I (MHC I) molecules on the cell surface. The overall quality of peptide-MHC I complexes (pMHC I) is determined by poorly understood mechanisms that generate and load peptides with appropriate consensus motifs onto MHC I. In this article, we show that both tapasin (Tpn), a key component of the peptide loading complex, and the endoplasmic reticulum aminopeptidase associated with Ag processing (ERAAP) are quintessential editors of distinct structural features of the peptide repertoire. We carried out reciprocal immunization of wild-type mice with cells from Tpn- or ERAAP-deficient mice. Specificity analysis of T cell responses showed that absence of Tpn or ERAAP independently altered the peptide repertoire by causing loss as well as gain of new pMHC I. Changes in amino acid sequences of MHC-bound peptides revealed that ERAAP and Tpn, respectively, defined the characteristic amino and carboxy termini of canonical MHC I peptides. Thus, the optimal pMHC I repertoire is produced by two distinct peptide editing steps in the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)1547-1555
Number of pages9
JournalJournal of Immunology
Issue number4
StatePublished - Aug 15 2013
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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