TY - JOUR
T1 - Epigenetic regulation of stem cell maintenance in the drosophila testis via the nucleosome-remodeling factor NURF
AU - Cherry, Christopher M.
AU - Matunis, Erika L.
N1 - Funding Information:
We thank our colleagues who have generously supplied us with reagents, Dr. K. Hohenstein for the initial characterization of nurf301 mutant larval gonads, and Dr. M. de Cuevas and M. Issigonis for comments on the manuscript. This work was supported by NIH grants HD052937 and HD040307-07 (E.L.M.).
PY - 2010/6/4
Y1 - 2010/6/4
N2 - Regulation of stem cells depends on both tissue-specific transcriptional regulators and changes in chromatin organization, yet the coordination of these events in endogenous niches is poorly understood. In the Drosophila testis, local JAK-STAT signaling maintains germline and somatic stem cells (GSCs and cyst progenitor cells, or CPCs) in a single niche. Here we show that epigenetic regulation via the nucleosome-remodeling factor (NURF) complex ensures GSC and CPC maintenance by positively regulating JAK-STAT signaling, thereby preventing premature differentiation. Conversely, NURF is not required in early differentiating daughter cells of either lineage. Because three additional ATP-dependent chromatin remodelers (ACF, CHRAC, and dMi-2/NuRD) are dispensable for stem cell maintenance in the testis, epigenetic regulation of stem cells within this niche may rely primarily on NURF. Thus, local signals cooperate with specific chromatin-remodeling complexes in intact niches to coordinately regulate a common set of target genes to prevent premature stem cell differentiation.
AB - Regulation of stem cells depends on both tissue-specific transcriptional regulators and changes in chromatin organization, yet the coordination of these events in endogenous niches is poorly understood. In the Drosophila testis, local JAK-STAT signaling maintains germline and somatic stem cells (GSCs and cyst progenitor cells, or CPCs) in a single niche. Here we show that epigenetic regulation via the nucleosome-remodeling factor (NURF) complex ensures GSC and CPC maintenance by positively regulating JAK-STAT signaling, thereby preventing premature differentiation. Conversely, NURF is not required in early differentiating daughter cells of either lineage. Because three additional ATP-dependent chromatin remodelers (ACF, CHRAC, and dMi-2/NuRD) are dispensable for stem cell maintenance in the testis, epigenetic regulation of stem cells within this niche may rely primarily on NURF. Thus, local signals cooperate with specific chromatin-remodeling complexes in intact niches to coordinately regulate a common set of target genes to prevent premature stem cell differentiation.
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U2 - 10.1016/j.stem.2010.04.018
DO - 10.1016/j.stem.2010.04.018
M3 - Article
C2 - 20569693
AN - SCOPUS:77956621313
SN - 1934-5909
VL - 6
SP - 557
EP - 567
JO - Cell stem cell
JF - Cell stem cell
IS - 6
ER -