Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection

Vasudev J. Bailey; Brian P. Keeley; Yi Zhang; Yi Ping Ho; Hariharan Easwaran; Malcolm V. Brock; Kristen L. Pelosky; Hetty E. Carraway; Stephen B. Baylin; James G. Herman; Tza Huei Wang

doi:10.1002/cbic.200900630

Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection

Vasudev J. Bailey, Brian P. Keeley, Yi Zhang, Yi Ping Ho, Hariharan Easwaran, Malcolm V. Brock, Kristen L. Pelosky, Hetty E. Carraway, Stephen B. Baylin, James G. Herman, Tza Huei Wang

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

(Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET).

Original language	English (US)
Pages (from-to)	71-74
Number of pages	4
Journal	ChemBioChem
Volume	11
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200900630
State	Published - Jan 4 2010

Keywords

Biosensors
DNA
Methylation
Optical analysis
Spectroscopic methods

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200900630

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title = "Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection",

abstract = "(Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET).",

keywords = "Biosensors, DNA, Methylation, Optical analysis, Spectroscopic methods",

author = "Bailey, {Vasudev J.} and Keeley, {Brian P.} and Yi Zhang and Ho, {Yi Ping} and Hariharan Easwaran and Brock, {Malcolm V.} and Pelosky, {Kristen L.} and Carraway, {Hetty E.} and Baylin, {Stephen B.} and Herman, {James G.} and Wang, {Tza Huei}",

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doi = "10.1002/cbic.200900630",

language = "English (US)",

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T1 - Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection

AU - Bailey, Vasudev J.

AU - Keeley, Brian P.

AU - Zhang, Yi

AU - Ho, Yi Ping

AU - Easwaran, Hariharan

AU - Brock, Malcolm V.

AU - Pelosky, Kristen L.

AU - Carraway, Hetty E.

AU - Baylin, Stephen B.

AU - Herman, James G.

AU - Wang, Tza Huei

PY - 2010/1/4

Y1 - 2010/1/4

N2 - (Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET).

AB - (Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET).

KW - Biosensors

KW - DNA

KW - Methylation

KW - Optical analysis

KW - Spectroscopic methods

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JO - ChemBioChem

JF - ChemBioChem

IS - 1

ER -

Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection

Abstract

Keywords

ASJC Scopus subject areas

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