Enzymatic Estimation of Urinary Steroids

Barbara Hurlock, Paul Talalay

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A novel method for the sensitive and specific microestimation of steroids has been described. This method depends upon the selective oxidation or reduction of respectively hydroxy- and ketosteroids by highly purified hydroxysteroid dehydrogenases of bacterial origin. The method permits the estimation of 3a-hydroxysteroids, 3β-(and 17β-) hydroxysteroids, 3-ketosteroids and 17-ketosteroids.

Original languageEnglish (US)
Pages (from-to)560-564
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number3
StatePublished - Dec 1956
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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