Enzymatic analysis of a rhomboid intramembrane protease implicates transmembrane helix 5 as the lateral substrate gate

Keyphrases

• Transmembrane Helix 100%
• Rhomboid Protease 100%
• Intramembrane Protease 100%
• Rhomboid 100%
• Enzymatic Analysis 100%
• Active Sites 66%
• Membrane Lipids 66%
• Gating Mechanism 66%
• Intramembrane Proteolysis 66%
• L1 Loops 66%
• Regulatory Mechanism 33%
• Functional Analysis 33%
• Protease 33%
• Mutagenesis 33%
• Hydrolysis 33%
• Structure Function 33%
• Hydrophobic Environment 33%
• Rate-limiting Step 33%
• Three-class 33%
• Transmembrane Segment 33%
• High-resolution Crystal Structure 33%
• Residue Interaction 33%
• Peptide Bond 33%
• Substrate Motif 33%
• Spitz 33%
• Substrate Entry 33%
• GlpG 33%
• Enhanced Enzyme Activity 33%

Biochemistry, Genetics and Molecular Biology

• Rhomboid Protease 100%
• Intramembrane Protease 100%
• Enzyme 66%
• Protein Catabolism 66%
• Active Site 66%
• Membrane Lipid 66%
• Crystal Structure 33%
• Protease 33%
• Enzyme Activity 33%
• Enzymatic Hydrolysis 33%
• Serine 33%
• Mutagenesis 33%
• Peptide Bond 33%