Enhancement of Antigen-Specific Activation of CD8+ Memory Cytotoxic T Cells by B Cell-Derived Factors

Ferdynand J. Kos, Arno Kos

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Purified CD8+ T cells from influenza A/WSN-immune BALB/c (H-2d) mice respond with the generation of secondary A/WSN-specific Tc cells in vitro when stimulated with a synthetic peptide (NPP) with a sequence derived from influenza A virus nucleoprotein with high affinity for Kd class I MHC molecules. The process of the conversion of NPP-Kd-responding Tc cell precursors into effector Tc cells in a population of CD8+ T cells occurs with no demonstrable requirements for accessory cells or their lymphokine products. The addition of culture supernatants from several mouse and human B cell lymphomas and LPS-activated normal mouse B cells to the culture of NPP-stimulated immune CD8+ T cells enhanced the induction of secondary Ag-specific Tc cells. None of the tested supernatants in the absence of Ag (NPP) induced cytolytic Tc cells, indicating that B cell-derived secretory factors can exert their activity only on Ag-exposed CD8+ T cells. The augmentatory effect of these supernatants on Ag-specific activation of memory CD8+ T cells was attributed to the synergism between B cell-derived factors and IL-2 which is produced endogenously in cultures of NPP-stimulated D8+ T cells. The possible role of B cell-derived helper factors is discussed.

Original languageEnglish (US)
Pages (from-to)410-420
Number of pages11
Issue number5
StatePublished - 1992


  • A/WSN
  • APC
  • Ag
  • B cell-derived culture supernatant(s)
  • B cell-derived factor(s)
  • BCDF
  • C'
  • EBV
  • Epstein-Barr virus
  • FBS
  • HAU
  • IL
  • LPS
  • NPP
  • Sup
  • Tc
  • antigen
  • antigen-presenting cell
  • complement
  • cytotoxic T (cell)
  • foetal bovine serum
  • hemagglutination unit
  • influenza virus reviations: Ag strain A/WSN/33 (HN)
  • interleukin
  • lipopolysaccharide
  • mAb
  • monoclonal antibody
  • synthetic influenza nucleoprotein peptide 147–158 R

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology


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