Abstract
Purified CD8+ T cells from influenza A/WSN-immune BALB/c (H-2d) mice respond with the generation of secondary A/WSN-specific Tc cells in vitro when stimulated with a synthetic peptide (NPP) with a sequence derived from influenza A virus nucleoprotein with high affinity for Kd class I MHC molecules. The process of the conversion of NPP-Kd-responding Tc cell precursors into effector Tc cells in a population of CD8+ T cells occurs with no demonstrable requirements for accessory cells or their lymphokine products. The addition of culture supernatants from several mouse and human B cell lymphomas and LPS-activated normal mouse B cells to the culture of NPP-stimulated immune CD8+ T cells enhanced the induction of secondary Ag-specific Tc cells. None of the tested supernatants in the absence of Ag (NPP) induced cytolytic Tc cells, indicating that B cell-derived secretory factors can exert their activity only on Ag-exposed CD8+ T cells. The augmentatory effect of these supernatants on Ag-specific activation of memory CD8+ T cells was attributed to the synergism between B cell-derived factors and IL-2 which is produced endogenously in cultures of NPP-stimulated D8+ T cells. The possible role of B cell-derived helper factors is discussed.
Original language | English (US) |
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Pages (from-to) | 410-420 |
Number of pages | 11 |
Journal | Immunobiology |
Volume | 186 |
Issue number | 5 |
DOIs | |
State | Published - 1992 |
Keywords
- A/WSN
- APC
- Ag
- B cell-derived culture supernatant(s)
- B cell-derived factor(s)
- BCDF
- C'
- EBV
- Epstein-Barr virus
- FBS
- HAU
- IL
- LPS
- NPP
- Sup
- Tc
- antigen
- antigen-presenting cell
- complement
- cytotoxic T (cell)
- foetal bovine serum
- hemagglutination unit
- influenza virus reviations: Ag strain A/WSN/33 (HN)
- interleukin
- lipopolysaccharide
- mAb
- monoclonal antibody
- synthetic influenza nucleoprotein peptide 147–158 R
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Hematology