Enhancement in rats by the liver tumor promoter ethinyl estradiol of a serum factor(s) which is stimulatory for hepatocyte DNA synthesis

Yuenian E. Shi; James D. Yager

doi:10.1016/0006-291X(89)91634-3

Enhancement in rats by the liver tumor promoter ethinyl estradiol of a serum factor(s) which is stimulatory for hepatocyte DNA synthesis

Yuenian E. Shi, James D. Yager

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Fractionation of female rat serum or plasma on Sephadex G-200 revealed the presence of an activity stimulatory for hepatocyte DNA synthesis. Treatment of female rats with the liver tumor promoter ethinyl estradiol (EE) at 2.5μg/day caused a 1.6 fold increase in the level of this activity at 24 hr in both serum and plasma. The stimulatory activity had a molecular weight of 135 kD, was sensitive to trypsin and heating and was not inhibited by the antiestrogen tamoxifen or antibody to epidermal growth factor (EGF). However, the pooled active fractions from EE-treated rats competed to a greater extent than comparable fractions from control rats for specific [¹²⁵I]-EGF binding to rat liver membranes. These results demonstrate that treatment of female rats with EE, under conditions known to stimulate liver growth, caused an increase in the level of a factor(s) stimulatory for hepatocyte DNA synthesis and whose activity may be mediated through the EGF receptor.

Original language	English (US)
Pages (from-to)	154-161
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)91634-3
State	Published - Apr 14 1989
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(89)91634-3

Cite this

@article{005084ec6b874316b5f76835e40828df,

title = "Enhancement in rats by the liver tumor promoter ethinyl estradiol of a serum factor(s) which is stimulatory for hepatocyte DNA synthesis",

abstract = "Fractionation of female rat serum or plasma on Sephadex G-200 revealed the presence of an activity stimulatory for hepatocyte DNA synthesis. Treatment of female rats with the liver tumor promoter ethinyl estradiol (EE) at 2.5μg/day caused a 1.6 fold increase in the level of this activity at 24 hr in both serum and plasma. The stimulatory activity had a molecular weight of 135 kD, was sensitive to trypsin and heating and was not inhibited by the antiestrogen tamoxifen or antibody to epidermal growth factor (EGF). However, the pooled active fractions from EE-treated rats competed to a greater extent than comparable fractions from control rats for specific [125I]-EGF binding to rat liver membranes. These results demonstrate that treatment of female rats with EE, under conditions known to stimulate liver growth, caused an increase in the level of a factor(s) stimulatory for hepatocyte DNA synthesis and whose activity may be mediated through the EGF receptor.",

author = "Shi, {Yuenian E.} and Yager, {James D.}",

note = "Funding Information: We wish to thank Dr. J. Zurlo for critical review of this manuscript. This work was supported by USPHS NC1 grant CA 36701 and Cancer Center Core Grant CA23108 to the Norris Cotton Cancer Center. YES was a receipient of a Friends of the Norris Cotton Cancer Center Predoctoral Fellowship.",

year = "1989",

month = apr,

day = "14",

doi = "10.1016/0006-291X(89)91634-3",

language = "English (US)",

volume = "160",

pages = "154--161",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Enhancement in rats by the liver tumor promoter ethinyl estradiol of a serum factor(s) which is stimulatory for hepatocyte DNA synthesis

AU - Shi, Yuenian E.

AU - Yager, James D.

N1 - Funding Information: We wish to thank Dr. J. Zurlo for critical review of this manuscript. This work was supported by USPHS NC1 grant CA 36701 and Cancer Center Core Grant CA23108 to the Norris Cotton Cancer Center. YES was a receipient of a Friends of the Norris Cotton Cancer Center Predoctoral Fellowship.

PY - 1989/4/14

Y1 - 1989/4/14

N2 - Fractionation of female rat serum or plasma on Sephadex G-200 revealed the presence of an activity stimulatory for hepatocyte DNA synthesis. Treatment of female rats with the liver tumor promoter ethinyl estradiol (EE) at 2.5μg/day caused a 1.6 fold increase in the level of this activity at 24 hr in both serum and plasma. The stimulatory activity had a molecular weight of 135 kD, was sensitive to trypsin and heating and was not inhibited by the antiestrogen tamoxifen or antibody to epidermal growth factor (EGF). However, the pooled active fractions from EE-treated rats competed to a greater extent than comparable fractions from control rats for specific [125I]-EGF binding to rat liver membranes. These results demonstrate that treatment of female rats with EE, under conditions known to stimulate liver growth, caused an increase in the level of a factor(s) stimulatory for hepatocyte DNA synthesis and whose activity may be mediated through the EGF receptor.

AB - Fractionation of female rat serum or plasma on Sephadex G-200 revealed the presence of an activity stimulatory for hepatocyte DNA synthesis. Treatment of female rats with the liver tumor promoter ethinyl estradiol (EE) at 2.5μg/day caused a 1.6 fold increase in the level of this activity at 24 hr in both serum and plasma. The stimulatory activity had a molecular weight of 135 kD, was sensitive to trypsin and heating and was not inhibited by the antiestrogen tamoxifen or antibody to epidermal growth factor (EGF). However, the pooled active fractions from EE-treated rats competed to a greater extent than comparable fractions from control rats for specific [125I]-EGF binding to rat liver membranes. These results demonstrate that treatment of female rats with EE, under conditions known to stimulate liver growth, caused an increase in the level of a factor(s) stimulatory for hepatocyte DNA synthesis and whose activity may be mediated through the EGF receptor.

UR - http://www.scopus.com/inward/record.url?scp=0024502161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024502161&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(89)91634-3

DO - 10.1016/0006-291X(89)91634-3

M3 - Article

C2 - 2785384

AN - SCOPUS:0024502161

SN - 0006-291X

VL - 160

SP - 154

EP - 161

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Enhancement in rats by the liver tumor promoter ethinyl estradiol of a serum factor(s) which is stimulatory for hepatocyte DNA synthesis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this