Abstract
The capability to rapidly and confidently determine or confirm the sequences of short oligonucleotides, including native and chemically-modified DNA and RNA, is important for a number of fields. While matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has been used previously to sequence short oligonucleotides, the typically low fragmentation efficiency of in-source or post-source decay processes necessitates the accumulation of a large number of spectra, thus limiting the throughput of these methods. Here we introduce a novel matrix, 1,5-diaminonapthalene (DAN), for facile in-source decay (ISD) of DNA and RNA molecular anions, which allows for rapid sequence confirmation. d-, w-, and yseries ions are prominent in the spectra, complementary to the (a-B)- and w- ions that are typically produced by MALDI post-source decay (PSD). Results are shown for several model DNA and RNA oligonucleotides, including combinations of DAN-induced fragmentation with true tandem TOF MS (MS/MS) for pseudo-MS 3 and "activated-ion PSD."
Original language | English (US) |
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Pages (from-to) | 773-777 |
Number of pages | 5 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 23 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2012 |
Externally published | Yes |
Keywords
- 1,5-Diaminonapthalene
- DNA
- In-Source decay
- Matrix-assisted laser desorption/ionization
- RNA
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy