Engineering the human blood-brain barrier in vitro

John J. Jamieson, Peter C. Searson, Sharon Gerecht

Research output: Contribution to journalReview articlepeer-review

Abstract

The blood-brain barrier (BBB) is the interface between the vasculature and the brain, regulating molecular and cellular transport into the brain. Endothelial cells (ECs) that form the capillary walls constitute the physical barrier but are dependent on interactions with other cell types. In vitro models are widely used in BBB research for mechanistic studies and drug screening. Current models have both biological and technical limitations. Here we review recent advances in stem cell engineering that have been utilized to create innovative platforms to replicate key features of the BBB. The development of human in vitro models is envisioned to enable new mechanistic investigations of BBB transport in central nervous system diseases.

Original languageEnglish (US)
Article number37
JournalJournal of Biological Engineering
Volume11
Issue number1
DOIs
StatePublished - Dec 4 2017
Externally publishedYes

Keywords

  • Blood-brain barrier
  • In vitro modeling
  • Induced pluripotent stem cells
  • Neurovascular unit

ASJC Scopus subject areas

  • Environmental Engineering
  • Biomedical Engineering
  • Molecular Biology
  • Cell Biology

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