Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes

Jong J. Kirn; Mark L. Bagarazzi; Neil Trivedi; Yin Hu; Ken Kazahaya; Darren M. Wilson; Richard Ciccarelli; Michael A. Chattergoon; Kesan Dang; Sundarasamy Mahalingam; Ara A. Chalian; Michael G. Agadjanyan; Jean D. Boyer; Bin Wang; David B. Weiner

doi:10.1038/nbt0797-641

Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes

Jong J. Kirn, Mark L. Bagarazzi, Neil Trivedi, Yin Hu, Ken Kazahaya, Darren M. Wilson, Richard Ciccarelli, Michael A. Chattergoon, Kesan Dang, Sundarasamy Mahalingam, Ara A. Chalian, Michael G. Agadjanyan, Jean D. Boyer, Bin Wang, David B. Weiner

Research output: Contribution to journal › Article › peer-review

209 Scopus citations

Abstract

Nucleic acid immunization is a novel vaccination technique to induce antigen-specific immune responses. We have developed expression cassettes for cell surface markers CD80 and CD86, two functionally related costimulatory molecules that play an important role in the induction of T cell-mediated immune responses. Coimmunization of these expression plasmids, along with plasmid DNA encoding for HIV-1 antigens, did not result in any significant change in the humoral response; however, we observed a dramatic increase in cytotoxic T-lymphocyte (CTL) induction as well as T-helper cell proliferation after the coadministration of CD86 genes, in contrast, coimmunization with a CD80 expression cassette resulted in a minor, but positive increase in T-helper cell or CTL responses. This strategy may be of value for the generation of rationally designed vaccines and immune therapeutics.

Original language	English (US)
Pages (from-to)	641-646
Number of pages	6
Journal	Nature biotechnology
Volume	15
Issue number	7
DOIs	https://doi.org/10.1038/nbt0797-641
State	Published - Jul 1997
Externally published	Yes

Keywords

Costimulatory molecules
DNA vaccines
Immune responses

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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Kirn, J. J., Bagarazzi, M. L., Trivedi, N., Hu, Y., Kazahaya, K., Wilson, D. M., Ciccarelli, R., Chattergoon, M. A., Dang, K., Mahalingam, S., Chalian, A. A., Agadjanyan, M. G., Boyer, J. D., Wang, B., & Weiner, D. B. (1997). Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes. Nature biotechnology, 15(7), 641-646. https://doi.org/10.1038/nbt0797-641

Kirn, JJ, Bagarazzi, ML, Trivedi, N, Hu, Y, Kazahaya, K, Wilson, DM, Ciccarelli, R, Chattergoon, MA, Dang, K, Mahalingam, S, Chalian, AA, Agadjanyan, MG, Boyer, JD, Wang, B & Weiner, DB 1997, 'Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes', Nature biotechnology, vol. 15, no. 7, pp. 641-646. https://doi.org/10.1038/nbt0797-641

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abstract = "Nucleic acid immunization is a novel vaccination technique to induce antigen-specific immune responses. We have developed expression cassettes for cell surface markers CD80 and CD86, two functionally related costimulatory molecules that play an important role in the induction of T cell-mediated immune responses. Coimmunization of these expression plasmids, along with plasmid DNA encoding for HIV-1 antigens, did not result in any significant change in the humoral response; however, we observed a dramatic increase in cytotoxic T-lymphocyte (CTL) induction as well as T-helper cell proliferation after the coadministration of CD86 genes, in contrast, coimmunization with a CD80 expression cassette resulted in a minor, but positive increase in T-helper cell or CTL responses. This strategy may be of value for the generation of rationally designed vaccines and immune therapeutics.",

keywords = "Costimulatory molecules, DNA vaccines, Immune responses",

author = "Kirn, {Jong J.} and Bagarazzi, {Mark L.} and Neil Trivedi and Yin Hu and Ken Kazahaya and Wilson, {Darren M.} and Richard Ciccarelli and Chattergoon, {Michael A.} and Kesan Dang and Sundarasamy Mahalingam and Chalian, {Ara A.} and Agadjanyan, {Michael G.} and Boyer, {Jean D.} and Bin Wang and Weiner, {David B.}",

year = "1997",

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doi = "10.1038/nbt0797-641",

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AU - Kazahaya, Ken

AU - Wilson, Darren M.

AU - Ciccarelli, Richard

AU - Chattergoon, Michael A.

AU - Dang, Kesan

AU - Mahalingam, Sundarasamy

AU - Chalian, Ara A.

AU - Agadjanyan, Michael G.

AU - Boyer, Jean D.

AU - Wang, Bin

AU - Weiner, David B.

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Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes

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