TY - JOUR
T1 - Endovascular model of rabbit hindlimb ischemia
T2 - A platform to evaluate therapeutic angiogenesis
AU - Liddell, Robert P.
AU - Patel, Tarak H.
AU - Weiss, Clifford R.
AU - Lee, David S.
AU - Matsuhashi, Toshio
AU - Brown, P. Rand
AU - Gabrielson, Kathleen L.
AU - Rodriguez, E. Rene
AU - Eng, John
AU - Kimura, Hideo
AU - Hofmann, Lawrence V.
PY - 2005/7
Y1 - 2005/7
N2 - PURPOSE: Current animal hindlimb ischemia models involve surgical ligation of the femoral artery and delivery of therapeutic angiogenic agents into the adductor compartment. The authors hypothesize that an endovascular model of hindlimb ischemia would be a more appropriate platform, closely resembling atherosclerosis by occluding the vessel from within, causing less inflammation, wound healing and subsequent collateralization. MATERIALS AND METHODS: The left superficial femoral artery in 17 rabbits was occluded by endovascular coil embolization (n = 9) or surgical ligation (n = 8). Animals (n = 3; in each group) were sacrificed on day 3 to determine the arteriolar luminal area, number of arterioles, microsphere determined perfusion, and degree of inflammation. On day 28, the remaining animals underwent calf blood pressure measurements and angiography to determine the number of collaterals and diameter of vessels supplying the hindlimb. RESULTS: Immediate postprocedure (day 0) and presacrifice (day 3 or 28) occlusion rates were 89% (eight of nine rabbits) and 100% for the endovascular model; 100% and 100% for the surgical model, respectively. Hindlimb paralysis and muscle atrophy was found in one surgical animal. On day 3, there was an increase in hindlimb perfusion (surgery, 0.04 ± 0.01; endovascular, 0.02 ± 0.01; P = .02), an increase in arteriolar luminal area (surgery, 481 μm ± 240; endovascular, 345 μm ± 151; P = .04), and a trend toward more inflammation (surgery, 5.5 ± 3.8; endovascular, 2.5 ± 3.0; P = .08) in the surgical group. There was no difference in dumber of vessels between both groups. On day 28 there was no difference in the calf blood pressure ratios or in the number of collaterals. However, there was enlargement of the distal profunda femoris artery, the vessel closest to the surgical incision, in the surgical group (L/R ratio: immediate post-occlusion, 1.06 ± 0.11; day 28,1.27 ± 0.08; P = .02). CONCLUSION: The endovascular model was efficacious in providing occlusion of the superficial femoral artery, and induced less of an arteriogenic response compared with the surgical model. The authors believe that this endovascular model is a superior platform for studying therapeutic angiogenic agents.
AB - PURPOSE: Current animal hindlimb ischemia models involve surgical ligation of the femoral artery and delivery of therapeutic angiogenic agents into the adductor compartment. The authors hypothesize that an endovascular model of hindlimb ischemia would be a more appropriate platform, closely resembling atherosclerosis by occluding the vessel from within, causing less inflammation, wound healing and subsequent collateralization. MATERIALS AND METHODS: The left superficial femoral artery in 17 rabbits was occluded by endovascular coil embolization (n = 9) or surgical ligation (n = 8). Animals (n = 3; in each group) were sacrificed on day 3 to determine the arteriolar luminal area, number of arterioles, microsphere determined perfusion, and degree of inflammation. On day 28, the remaining animals underwent calf blood pressure measurements and angiography to determine the number of collaterals and diameter of vessels supplying the hindlimb. RESULTS: Immediate postprocedure (day 0) and presacrifice (day 3 or 28) occlusion rates were 89% (eight of nine rabbits) and 100% for the endovascular model; 100% and 100% for the surgical model, respectively. Hindlimb paralysis and muscle atrophy was found in one surgical animal. On day 3, there was an increase in hindlimb perfusion (surgery, 0.04 ± 0.01; endovascular, 0.02 ± 0.01; P = .02), an increase in arteriolar luminal area (surgery, 481 μm ± 240; endovascular, 345 μm ± 151; P = .04), and a trend toward more inflammation (surgery, 5.5 ± 3.8; endovascular, 2.5 ± 3.0; P = .08) in the surgical group. There was no difference in dumber of vessels between both groups. On day 28 there was no difference in the calf blood pressure ratios or in the number of collaterals. However, there was enlargement of the distal profunda femoris artery, the vessel closest to the surgical incision, in the surgical group (L/R ratio: immediate post-occlusion, 1.06 ± 0.11; day 28,1.27 ± 0.08; P = .02). CONCLUSION: The endovascular model was efficacious in providing occlusion of the superficial femoral artery, and induced less of an arteriogenic response compared with the surgical model. The authors believe that this endovascular model is a superior platform for studying therapeutic angiogenic agents.
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U2 - 10.1097/01.RVI.0000161381.48445.48
DO - 10.1097/01.RVI.0000161381.48445.48
M3 - Article
C2 - 16002507
AN - SCOPUS:22344438897
SN - 1051-0443
VL - 16
SP - 991
EP - 998
JO - Journal of Vascular and Interventional Radiology
JF - Journal of Vascular and Interventional Radiology
IS - 7
ER -