TY - JOUR
T1 - Endothelin 1
T2 - Mitogenic Activity on Pulmonary Artery Smooth Muscle Cells and Release from Hypoxic Endothelial Cells
AU - Hassoun, P. M.
AU - Thappa, V.
AU - Landman, M. J.
AU - Fanburg, B. L.
PY - 1992/2
Y1 - 1992/2
N2 - Endothelin, a recently described vasoconstrictor, has been shown to be a mitogen for vascular smooth muscle cells from systemic arteries and might play a role in pulmonary vascular remodeling produced by chronic exposure to hypoxia. We examined the effects of endothelin on proliferation of pulmonary artery smooth muscle cells and the effects of hypoxia and normoxia on the synthesis and secretion of endothelin by endothelial cells. Our results indicate that endothelin significantly increased the incorporation of [3H]thymidine by porcine pulmonary artery smooth muscle cells (122 ± 4% to 168 ± 13% of controls, with concentrations of endothelin from 1 to 1000 ng/ml). When tested on bovine pulmonary artery smooth muscle cells, endothelin increased [3H]thymidine incorporation over controls by approximately 140%; cell counts were increased by 107 ± 4% and 122 ± 7% at doses of 100 ng/ml and 1000 ng/ml, respectively. The secretion of endothelin by porcine endothelial cells was not affected by hypoxia (3520 ± 138 pg/ml/106 cells in hypoxia vs 3770 ± 326 pg/ml/106 cells in normoxia). Transforming growth factor-β1 stimulated the release by normoxic, and to a lesser degree by hypoxic, porcine endothelial cells of endothelin (4716 ± 43 pg/ml/106 cells vs 4074 ± 106 pg/ml/106 cells). Taken together, our results indicate that endothelin is weakly mitogenic for pulmonary artery smooth muscle cells, but may not significantly contribute to the remodeling seen in hypoxic pulmonary hypertension.
AB - Endothelin, a recently described vasoconstrictor, has been shown to be a mitogen for vascular smooth muscle cells from systemic arteries and might play a role in pulmonary vascular remodeling produced by chronic exposure to hypoxia. We examined the effects of endothelin on proliferation of pulmonary artery smooth muscle cells and the effects of hypoxia and normoxia on the synthesis and secretion of endothelin by endothelial cells. Our results indicate that endothelin significantly increased the incorporation of [3H]thymidine by porcine pulmonary artery smooth muscle cells (122 ± 4% to 168 ± 13% of controls, with concentrations of endothelin from 1 to 1000 ng/ml). When tested on bovine pulmonary artery smooth muscle cells, endothelin increased [3H]thymidine incorporation over controls by approximately 140%; cell counts were increased by 107 ± 4% and 122 ± 7% at doses of 100 ng/ml and 1000 ng/ml, respectively. The secretion of endothelin by porcine endothelial cells was not affected by hypoxia (3520 ± 138 pg/ml/106 cells in hypoxia vs 3770 ± 326 pg/ml/106 cells in normoxia). Transforming growth factor-β1 stimulated the release by normoxic, and to a lesser degree by hypoxic, porcine endothelial cells of endothelin (4716 ± 43 pg/ml/106 cells vs 4074 ± 106 pg/ml/106 cells). Taken together, our results indicate that endothelin is weakly mitogenic for pulmonary artery smooth muscle cells, but may not significantly contribute to the remodeling seen in hypoxic pulmonary hypertension.
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U2 - 10.3181/00379727-199-43342
DO - 10.3181/00379727-199-43342
M3 - Article
C2 - 1741408
AN - SCOPUS:0026531570
SN - 0037-9727
VL - 199
SP - 165
EP - 170
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 2
ER -