@article{a98812db84674bedbed24ea48e4c0743,
title = "Endothelial infection with KSHV genes in vivo reveals that vGPCR initiates Kaposi's sarcomagenesis and can promote the tumorigenic potential of viral latent genes",
abstract = "The Kaposi's sarcoma herpesvirus (KSHV) has been identified as the etiologic agent of Kaposi's sarcoma (KS), but initial events leading to KS development remain unclear. Characterization of the KSHV genome reveals the presence of numerous potential oncogenes. To address their contribution to the initiation of the endothelial cell-derived KS tumor, we developed a novel transgenic mouse that enabled endothelial cell-specific infection in vivo using virus expressing candidate KSHV oncogenes. Here we show that transduction of one gene, vGPCR, was sufficient to induce angioproliferative tumors that strikingly resembled human KS. Endothelial cells expressing vGPCR were further able to promote tumor formation by cells expressing KSHV latent genes, suggestive of a cooperative role among viral genes in the promotion of Kaposi's sarcomagenesis.",
author = "Silvia Montaner and Akrit Sodhi and Alfredo Molinolo and Bugge, {Thomas H.} and Sawai, {Earl T.} and Yunsheng He and Yi Li and Ray, {Patricio E.} and Gutkind, {J. Silvio}",
note = "Funding Information: We thank Harold E. Varmus (MSKCC) for insightful suggestions and discussion, Thomas N. Sato (University of Texas) for providing the plasmid containing the TIE2 promoter-enhancer, Steve Hughes (FCRC, NCI, NIH) for the RCAS and RCAS- AP plasmids, Doug Foster (University of Minnesota) for the DF-1 cell line, Andrew Leavitt (UCSF) for the TVA antibody, the Gene Targeting Core Facility (NIDCR) for technical assistance with the development of the transgenic line, Martin Kriete and the Veterinary Resources Core Facility (NIDCR) for assistance with the animal care, SAIC Frederick for tissue preparation and immunohistochemical staining, Keith Rogers for technical assistance, Miriam Anvers for assistance with the histological analysis of pathology samples, and William D. Swaim for assistance with the electron microscopy. This work has been supported by NIH grants 2RO-1 DK 49419, 2RO-1 HL 55605 (PER), and RO-1 AI46145-01A2 and a grant from the Department of Defense, BC972195.",
year = "2003",
month = jan,
doi = "10.1016/S1535-6108(02)00237-4",
language = "English (US)",
volume = "3",
pages = "23--36",
journal = "Cancer cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "1",
}