Endothelial cell Ca2+ is increased by melanoma cell adhesion

R. Pili, R. C. Ziegelstein, A. Passaniti, M. C. Capogrossi

Research output: Contribution to journalArticlepeer-review


The signal transduction mechanisms that control tumor cell-endothelium adhesion and tumor cell migration are still largely undefined. We examined the effect of metastatic murine melanoma cell (B16/ G3.26) adhesion on cytosolic [Ca2+] (Ca(i)) of bovine pulmonary artery endothelial cells (BPAECs), loaded with the membrane-permeant ester form of the fluorescent Ca2+ indicator indo-1. Indo-1 was excited at 350 nm and the ratio of emitted indo-1 fluorescence at 410/490 nm was used as an index of Ca(i),. Upon contact between melanoma and endothelial cells there was a rapid increase in endothelial cell Ca(i) which exhibited a partial recovery toward control. BPAECs contact with 8 μm inert beads did not elicit an increase in Ca. In conclusion, melanoma-endothelium contact modulates endothelial cell Ca(i); homeostasis.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalClinical Chemistry and Enzymology Communications
Issue number1-2
StatePublished - Dec 1 1993
Externally publishedYes


  • Adhesion
  • Calcium
  • Endothelium
  • Melanoma

ASJC Scopus subject areas

  • Clinical Biochemistry


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