Endonucleolytic activity for γ-irradiated DNA in normal and ataxia telangiectasia fibroblast cell extracts

R. B. Sheridan; P. C. Huang

doi:10.1016/0027-5107(79)90070-8

Endonucleolytic activity for γ-irradiated DNA in normal and ataxia telangiectasia fibroblast cell extracts

R. B. Sheridan
, P. C. Huang

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The increased sensitivity of ataxia telangiectasia cells towards ionizing radiation may be related to their inability to incise DNA near sites of radiation-induced base damages. When compared to 3 unaffected controls, crude extracts from 5 lines of fibroblast cells derived from ataxia telangiectasia patients were capable of incising γ-irradiated DNA to the same extent as normal cells as determined in a nicking assay, using the circular replicative form of φX174. However, the types of alterations introduced into DNA by γ-irradiation could be distinguished from sites of base loss due to depurination or depyrimidination and from sites of base modification by OsO₄. The specific endonuclease involved was demonstrated to be distinct from the apurinic endonuclease by its rate of temperature inactivation.

Original language	English (US)
Pages (from-to)	387-392
Number of pages	6
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0027-5107(79)90070-8
State	Published - Dec 1979
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/0027-5107(79)90070-8

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title = "Endonucleolytic activity for γ-irradiated DNA in normal and ataxia telangiectasia fibroblast cell extracts",

abstract = "The increased sensitivity of ataxia telangiectasia cells towards ionizing radiation may be related to their inability to incise DNA near sites of radiation-induced base damages. When compared to 3 unaffected controls, crude extracts from 5 lines of fibroblast cells derived from ataxia telangiectasia patients were capable of incising γ-irradiated DNA to the same extent as normal cells as determined in a nicking assay, using the circular replicative form of φX174. However, the types of alterations introduced into DNA by γ-irradiation could be distinguished from sites of base loss due to depurination or depyrimidination and from sites of base modification by OsO4. The specific endonuclease involved was demonstrated to be distinct from the apurinic endonuclease by its rate of temperature inactivation.",

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T1 - Endonucleolytic activity for γ-irradiated DNA in normal and ataxia telangiectasia fibroblast cell extracts

AU - Sheridan, R. B.

AU - Huang, P. C.

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Y1 - 1979/12

N2 - The increased sensitivity of ataxia telangiectasia cells towards ionizing radiation may be related to their inability to incise DNA near sites of radiation-induced base damages. When compared to 3 unaffected controls, crude extracts from 5 lines of fibroblast cells derived from ataxia telangiectasia patients were capable of incising γ-irradiated DNA to the same extent as normal cells as determined in a nicking assay, using the circular replicative form of φX174. However, the types of alterations introduced into DNA by γ-irradiation could be distinguished from sites of base loss due to depurination or depyrimidination and from sites of base modification by OsO4. The specific endonuclease involved was demonstrated to be distinct from the apurinic endonuclease by its rate of temperature inactivation.

AB - The increased sensitivity of ataxia telangiectasia cells towards ionizing radiation may be related to their inability to incise DNA near sites of radiation-induced base damages. When compared to 3 unaffected controls, crude extracts from 5 lines of fibroblast cells derived from ataxia telangiectasia patients were capable of incising γ-irradiated DNA to the same extent as normal cells as determined in a nicking assay, using the circular replicative form of φX174. However, the types of alterations introduced into DNA by γ-irradiation could be distinguished from sites of base loss due to depurination or depyrimidination and from sites of base modification by OsO4. The specific endonuclease involved was demonstrated to be distinct from the apurinic endonuclease by its rate of temperature inactivation.

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Endonucleolytic activity for γ-irradiated DNA in normal and ataxia telangiectasia fibroblast cell extracts

Abstract

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