TY - JOUR
T1 - Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
AU - Barletta, Ana Beatriz Ferreira
AU - Alves, Liliane Rosa
AU - Nascimento Silva, Maria Clara L.
AU - Sim, Shuzhen
AU - Dimopoulos, George
AU - Liechocki, Sally
AU - Maya-Monteiro, Clarissa M.
AU - Sorgine, Marcos H.Ferreira
N1 - Funding Information:
We thank Katia Anastácio Laia and Jaciara Pingo for rearing insects, S.R. Cassia for technical assistance. Dr. Pedro Lagerblad de Oliveira for scientific advice and Dr. Felipe de Almeida Dias for E. cloacae and M. luteus used in the experiments. We also thank Dr. Thiago Luiz Alves e Silva for the assistance in manuscript preparation. We thank the Conselho Nacional de Tecnologia (CNPq), Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular (INCT-EM) for financial support. We thank Johns Hopkins Malaria Research Institute Genome Core Facility for assistance microarray analyses. This work has been supported by the National Institutes of Health/National Institute for Allergy and Infectious Disease grants R01AI061576 and R01AI081877.
PY - 2016/2/18
Y1 - 2016/2/18
N2 - In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells-an immune responsive cell lineage-accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism.
AB - In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells-an immune responsive cell lineage-accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism.
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U2 - 10.1038/srep19928
DO - 10.1038/srep19928
M3 - Article
C2 - 26887863
AN - SCOPUS:84959018786
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 19928
ER -