ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates

Sergei A. Tsarev; Tatiana S. Tsareva; Suzanne U. Emerson; Albert Z. Kapikian; John Ticehurst; William London; Robert H. Purcell

doi:10.1093/infdis/168.2.369

ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates

Sergei A. Tsarev, Tatiana S. Tsareva, Suzanne U. Emerson, Albert Z. Kapikian, John Ticehurst, William London, Robert H. Purcell

Research output: Contribution to journal › Article › peer-review

202 Scopus citations

Abstract

A recombinant baculovirus containing the complete open-reading frame (ORF)-2 region of the hepatitis E virus (HEV) genome was constructed. The major protein synthesized in insect cells infected with recombinant virus was about the size expected for the complete ORF-2 product. This protein reacted in a Western blot assay with plasma from an HEV-infected chimpanzee. Lysates of the recombinant virus-infected insect cells were used in ELISA to monitor seroconversion of eight primate species (chimpanzees, four species of Old World monkeys, and three species ofNew World monkeys) inoculated with HEV. Homologous detector anti-immunoglobulin was more sensitive than heterologous anti-immunoglobulin for detecting anti-HEV by ELISA. All primate species except tamarins seroconverted after inoculation with HEV, although anti-HEV titers of Old World monkey species were generally higher than those of New World monkey species. The ELISA with complete ORF-2 antigen appeared to be a sensitive and practical method for detecting anti-HEV.

Original language	English (US)
Pages (from-to)	369-378
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	168
Issue number	2
DOIs	https://doi.org/10.1093/infdis/168.2.369
State	Published - Aug 1993
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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Tsarev, S. A., Tsareva, T. S., Emerson, S. U., Kapikian, A. Z., Ticehurst, J., London, W., & Purcell, R. H. (1993). ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates. Journal of Infectious Diseases, 168(2), 369-378. https://doi.org/10.1093/infdis/168.2.369

ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates. / Tsarev, Sergei A.; Tsareva, Tatiana S.; Emerson, Suzanne U. et al.
In: Journal of Infectious Diseases, Vol. 168, No. 2, 08.1993, p. 369-378.

Research output: Contribution to journal › Article › peer-review

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abstract = "A recombinant baculovirus containing the complete open-reading frame (ORF)-2 region of the hepatitis E virus (HEV) genome was constructed. The major protein synthesized in insect cells infected with recombinant virus was about the size expected for the complete ORF-2 product. This protein reacted in a Western blot assay with plasma from an HEV-infected chimpanzee. Lysates of the recombinant virus-infected insect cells were used in ELISA to monitor seroconversion of eight primate species (chimpanzees, four species of Old World monkeys, and three species ofNew World monkeys) inoculated with HEV. Homologous detector anti-immunoglobulin was more sensitive than heterologous anti-immunoglobulin for detecting anti-HEV by ELISA. All primate species except tamarins seroconverted after inoculation with HEV, although anti-HEV titers of Old World monkey species were generally higher than those of New World monkey species. The ELISA with complete ORF-2 antigen appeared to be a sensitive and practical method for detecting anti-HEV.",

author = "Tsarev, {Sergei A.} and Tsareva, {Tatiana S.} and Emerson, {Suzanne U.} and Kapikian, {Albert Z.} and John Ticehurst and William London and Purcell, {Robert H.}",

note = "Funding Information: Received 4 December 1992; revised 29 March 1993. Primates were individually housed under BL-2 biohazard containment. The housing. maintenance. and care of the animals met or exceeded all requirements for primate husbandry. The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views ofthe US Department of the Army or the Department of Defense. Financial support: World Health Organization Programme for Vaccine Development; National Institutes of Health (AI-05069). Reprints or correspondence: Dr. Sergei Tsarev, LID/NIAID/NIH, Bldg. 7. Rm. 200, 9000 Rockville Pike, Bethesda, MD 20892.",

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ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates

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