Elimination of mycoplasma from human B-lymphoblastoid cell lines

David N. Howell; Carolyn E. Machamer; Peter Cresswell

doi:10.1016/0198-8859(82)90136-7

Elimination of mycoplasma from human B-lymphoblastoid cell lines

David N. Howell, Carolyn E. Machamer, Peter Cresswell

Research output: Contribution to journal › Article › peer-review

Abstract

Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all cases, nude mouse passage for as little as 5 days was totally effective in removing all contaminating mycoplasma.

Original language	English (US)
Pages (from-to)	233-238
Number of pages	6
Journal	Human Immunology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/0198-8859(82)90136-7
State	Published - Nov 1982
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0198-8859(82)90136-7

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title = "Elimination of mycoplasma from human B-lymphoblastoid cell lines",

abstract = "Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all cases, nude mouse passage for as little as 5 days was totally effective in removing all contaminating mycoplasma.",

author = "Howell, {David N.} and Machamer, {Carolyn E.} and Peter Cresswell",

note = "Funding Information: This work was supported by NIH Grants AI-15775 and A1-14016. David Howell is supported by NIH-MSTP Grant 5 T32 GMO7171. We thank Ms. Susan Poulton for providing much useful information on the mainten~ce and use of nude mice, Dr. Donna Kostyu for performing microcytotoxicity typings, and Mrs. Jackie Smith for typing the manuscript.",

year = "1982",

month = nov,

doi = "10.1016/0198-8859(82)90136-7",

language = "English (US)",

volume = "5",

pages = "233--238",

journal = "Human Immunology",

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T1 - Elimination of mycoplasma from human B-lymphoblastoid cell lines

AU - Howell, David N.

AU - Machamer, Carolyn E.

AU - Cresswell, Peter

N1 - Funding Information: This work was supported by NIH Grants AI-15775 and A1-14016. David Howell is supported by NIH-MSTP Grant 5 T32 GMO7171. We thank Ms. Susan Poulton for providing much useful information on the mainten~ce and use of nude mice, Dr. Donna Kostyu for performing microcytotoxicity typings, and Mrs. Jackie Smith for typing the manuscript.

PY - 1982/11

Y1 - 1982/11

N2 - Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all cases, nude mouse passage for as little as 5 days was totally effective in removing all contaminating mycoplasma.

AB - Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all cases, nude mouse passage for as little as 5 days was totally effective in removing all contaminating mycoplasma.

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JO - Human Immunology

JF - Human Immunology

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ER -

Elimination of mycoplasma from human B-lymphoblastoid cell lines

Abstract

ASJC Scopus subject areas

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