Elimination of mRNA splicing by a point mutation outside the conserved GU at 5′ splice sites

Craig Montell, Arnold J. Berk

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5′ splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5′ splice site. Here we show that a single A→11 transversion in the third position of the adenovirus? F1A 13S mRNA intron does prevent RNA splicing at the corresponding 5′ splice site.

Original languageEnglish (US)
Pages (from-to)3821-3827
Number of pages7
JournalNucleic Acids Research
Volume12
Issue number9
DOIs
StatePublished - May 11 1984
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Fingerprint

Dive into the research topics of 'Elimination of mRNA splicing by a point mutation outside the conserved GU at 5′ splice sites'. Together they form a unique fingerprint.

Cite this