Electrophoretic separation of human embryonic globin demonstrates "α-thalassemia" in human leukemia cell line K562

Blanche P. Alter; Sabra C. Goff

doi:10.1016/0006-291X(80)91311-X

Electrophoretic separation of human embryonic globin demonstrates "α-thalassemia" in human leukemia cell line K562

Blanche P. Alter
, Sabra C. Goff

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Human embryonic, fetal, and adult globin chains (ζ, ε, ^Aγ, ^Gγ, β, α) can be separated by electrophoresis on Triton Acid urea gels. K562, a human leukemia cell line, was induced with hemin, labelled with [³H]-leucine, and globin synthesis analyzed. All globins except β were produced. ε > ζ; ^Gγ:^Aγ=70:30; non-α:α=>2:1. Thus, hemin-induced K562 synthesized embryonic and fetal globin chains, and had globin synthetic imbalance, with "α-thalassemia.".

Original language	English (US)
Pages (from-to)	843-848
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(80)91311-X
State	Published - Jun 16 1980
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0006-291X(80)91311-X

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@article{4f43312c1f094a038b7c863a69d20482,

title = "Electrophoretic separation of human embryonic globin demonstrates {"}α-thalassemia{"} in human leukemia cell line K562",

abstract = "Human embryonic, fetal, and adult globin chains (ζ, ε, Aγ, Gγ, β, α) can be separated by electrophoresis on Triton Acid urea gels. K562, a human leukemia cell line, was induced with hemin, labelled with [3H]-leucine, and globin synthesis analyzed. All globins except β were produced. ε > ζ; Gγ:Aγ=70:30; non-α:α=>2:1. Thus, hemin-induced K562 synthesized embryonic and fetal globin chains, and had globin synthetic imbalance, with {"}α-thalassemia.{"}.",

author = "Alter, \{Blanche P.\} and Goff, \{Sabra C.\}",

year = "1980",

month = jun,

day = "16",

doi = "10.1016/0006-291X(80)91311-X",

language = "English (US)",

volume = "94",

pages = "843--848",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Electrophoretic separation of human embryonic globin demonstrates "α-thalassemia" in human leukemia cell line K562

AU - Alter, Blanche P.

AU - Goff, Sabra C.

PY - 1980/6/16

Y1 - 1980/6/16

N2 - Human embryonic, fetal, and adult globin chains (ζ, ε, Aγ, Gγ, β, α) can be separated by electrophoresis on Triton Acid urea gels. K562, a human leukemia cell line, was induced with hemin, labelled with [3H]-leucine, and globin synthesis analyzed. All globins except β were produced. ε > ζ; Gγ:Aγ=70:30; non-α:α=>2:1. Thus, hemin-induced K562 synthesized embryonic and fetal globin chains, and had globin synthetic imbalance, with "α-thalassemia.".

AB - Human embryonic, fetal, and adult globin chains (ζ, ε, Aγ, Gγ, β, α) can be separated by electrophoresis on Triton Acid urea gels. K562, a human leukemia cell line, was induced with hemin, labelled with [3H]-leucine, and globin synthesis analyzed. All globins except β were produced. ε > ζ; Gγ:Aγ=70:30; non-α:α=>2:1. Thus, hemin-induced K562 synthesized embryonic and fetal globin chains, and had globin synthetic imbalance, with "α-thalassemia.".

UR - https://www.scopus.com/pages/publications/0019313139

UR - https://www.scopus.com/pages/publications/0019313139#tab=citedBy

U2 - 10.1016/0006-291X(80)91311-X

DO - 10.1016/0006-291X(80)91311-X

M3 - Article

C2 - 6930971

AN - SCOPUS:0019313139

SN - 0006-291X

VL - 94

SP - 843

EP - 848

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Electrophoretic separation of human embryonic globin demonstrates "α-thalassemia" in human leukemia cell line K562

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