TY - JOUR
T1 - Electron paramagnetic resonance oxygen mapping (EPROM)
T2 - Direct visualization of oxygen concentration in tissue
AU - Velan, S. Sendhil
AU - Spencer, Richard G S
AU - Zweier, Jay L.
AU - Kuppusamy, Periannan
PY - 2000
Y1 - 2000
N2 - Tissue oxygen content is a central parameter in physiology but is difficult to measure. We report a novel procedure for spatial mapping of oxygen by electron paramagnetic resonance (EPR) utilizing a spectral-spatial imaging data set, in which an EPR spectrum is obtained from each image volume element. From this data set, spatial maps corresponding to local spin density and maximum EPR spectral line amplitude are generated. A map of local EPR spectral linewidth is then computed. Because linewidth directly correlates with oxygen concentration, the linewidth image provides a map of oxygenation. This method avoids a difficulty inherent in other oxygen content mapping techniques using EPR, that is, the unwanted influence of local spin probe density on the image. We provide simulation results and data from phantom studies demonstrating the validity of this method. We then apply the method to map oxygen content in rat tail tissue and vasculature. This method provides a new, widely applicable, approach to direct visualization of oxygen concentration in living tissue. (C) 2000 Wiley-Liss, Inc.
AB - Tissue oxygen content is a central parameter in physiology but is difficult to measure. We report a novel procedure for spatial mapping of oxygen by electron paramagnetic resonance (EPR) utilizing a spectral-spatial imaging data set, in which an EPR spectrum is obtained from each image volume element. From this data set, spatial maps corresponding to local spin density and maximum EPR spectral line amplitude are generated. A map of local EPR spectral linewidth is then computed. Because linewidth directly correlates with oxygen concentration, the linewidth image provides a map of oxygenation. This method avoids a difficulty inherent in other oxygen content mapping techniques using EPR, that is, the unwanted influence of local spin probe density on the image. We provide simulation results and data from phantom studies demonstrating the validity of this method. We then apply the method to map oxygen content in rat tail tissue and vasculature. This method provides a new, widely applicable, approach to direct visualization of oxygen concentration in living tissue. (C) 2000 Wiley-Liss, Inc.
KW - EPR
KW - EPR imaging
KW - Nitroxide
KW - Oximetry
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U2 - 10.1002/1522-2594(200006)43:6<804::AID-MRM5>3.0.CO;2-B
DO - 10.1002/1522-2594(200006)43:6<804::AID-MRM5>3.0.CO;2-B
M3 - Article
C2 - 10861874
AN - SCOPUS:0034089501
SN - 0740-3194
VL - 43
SP - 804
EP - 809
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
IS - 6
ER -