TY - JOUR
T1 - Electrolyte transport in piglets infected with transmissible gastroenteritis virus. Stimulation by verapamil and clonidine
AU - Homaidan, Fadia R.
AU - Torres, Alfonso
AU - Donowitz, Mark
AU - Sharp, Geoffrey W.G.
PY - 1991/10
Y1 - 1991/10
N2 - The effects of clonidine, an α2-adrenergic agonist, and verapamil, a Ca2+ channel blocker, on Na2+ and Cl- absorption were studied in stripped jejunal mucosa from control and transmissible-gastroenteritis-virus-infected piglets. All infected piglets developed severe diarrhea 18-24 hours after oral inoculation. Jejunum from infected animals, as compared with control jejunum, had decreased mucosal-to-serosal, serosal-to-mucosal, and net Na+ and Cl- fluxes. Clonidine and verapamil caused a decrease in short-circuit current and stimulation of Na+ and Cl- absorption in control jejunum. In infected piglets, although the jejunum exhibited severe villus atrophy, both drugs stimulated Na+ and Cl- absorption and the magnitude of Na+ and Cl- absorption was similar in control and transmissible-gastroenteritis-infected jejunum. In contrast, d-glucose stimulated Na+ absorption, and the decrease in shortcircuit current caused by verapamil and clonidine, were decreased in transmissible-gastroenteritis-infected jejunum. Such pharmacological stimulation of Na+ and Cl- absorption might be useful in the management and treatment of certain viral diarrheal diseases.
AB - The effects of clonidine, an α2-adrenergic agonist, and verapamil, a Ca2+ channel blocker, on Na2+ and Cl- absorption were studied in stripped jejunal mucosa from control and transmissible-gastroenteritis-virus-infected piglets. All infected piglets developed severe diarrhea 18-24 hours after oral inoculation. Jejunum from infected animals, as compared with control jejunum, had decreased mucosal-to-serosal, serosal-to-mucosal, and net Na+ and Cl- fluxes. Clonidine and verapamil caused a decrease in short-circuit current and stimulation of Na+ and Cl- absorption in control jejunum. In infected piglets, although the jejunum exhibited severe villus atrophy, both drugs stimulated Na+ and Cl- absorption and the magnitude of Na+ and Cl- absorption was similar in control and transmissible-gastroenteritis-infected jejunum. In contrast, d-glucose stimulated Na+ absorption, and the decrease in shortcircuit current caused by verapamil and clonidine, were decreased in transmissible-gastroenteritis-infected jejunum. Such pharmacological stimulation of Na+ and Cl- absorption might be useful in the management and treatment of certain viral diarrheal diseases.
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U2 - 10.1016/0016-5085(91)90713-U
DO - 10.1016/0016-5085(91)90713-U
M3 - Article
C2 - 1889713
AN - SCOPUS:0026000187
SN - 0016-5085
VL - 101
SP - 895
EP - 901
JO - Gastroenterology
JF - Gastroenterology
IS - 4
ER -