Electroconductive Nanopatterned Substrates for Enhanced Myogenic Differentiation and Maturation

Hee Seok Yang, Bora Lee, Jonathan H. Tsui, Jesse Macadangdang, Seok Young Jang, Sung Gap Im, Deok Ho Kim

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Electrically conductive materials provide a suitable platform for the in vitro study of excitable cells, such as skeletal muscle cells, due to their inherent conductivity and electroactivity. Here it is demonstrated that bioinspired electroconductive nanopatterned substrates enhance myogenic differentiation and maturation. The topographical cues from the highly aligned collagen bundles that form the extracellular matrix of skeletal muscle tissue are mimicked using nanopatterns created with capillary force lithography. Electron beam deposition is then utilized to conformally coat nanopatterned substrates with a thin layer of either gold or titanium to create electroconductive substrates with well-defined, large-area nanotopographical features. C2C12 cells, a myoblast cell line, are cultured for 7 d on substrates and the effects of topography and electrical conductivity on cellular morphology and myogenic differentiation are assessed. It is found that biomimetic nanotopography enhances the formation of aligned myotubes and the addition of an electroconductive coating promotes myogenic differentiation and maturation, as indicated by the upregulation of myogenic regulatory factors Myf5, MyoD, and myogenin (MyoG). These results suggest the suitability of electroconductive nanopatterned substrates as a biomimetic platform for the in vitro engineering of skeletal muscle tissue.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalAdvanced Healthcare Materials
Issue number1
StatePublished - Jan 1 2016
Externally publishedYes


  • Electroconductive substrates
  • Maturation
  • Myogenic differentiation
  • Nanotopography
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science


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