TY - CHAP
T1 - Electrochemical Aptamer-Based Sensors
T2 - A Platform Approach to High-Frequency Molecular Monitoring In Situ in the Living Body
AU - Dauphin-Ducharme, Philippe
AU - Ploense, Kyle L.
AU - Arroyo-Currás, Netzahualcóyotl
AU - Kippin, Tod E.
AU - Plaxco, Kevin W.
N1 - Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - The monitoring of specific molecules in the living body has historically required sample removal (e.g., blood draws, microdialysis) followed by analysis via cumbersome, laboratory-bound processes. Those few exceptions to this rule (e.g., glucose, pyruvate, the monoamines) are monitored using “one-off” technologies reliant on the specific enzymatic or redox reactivity of their targets, and thus not generalizable to the measurement of other targets. In response we have developed in vivo electrochemical aptamer-based (E-AB) sensors, a modular, receptor-based measurement technology that is independent of the chemical reactivity of its targets, and thus has the potential to be generalizable to a wide range of analytes. To further the adoption of this in vivo molecular measurement approach by other researchers and to accelerate its ultimate translation to the clinic, we present here our standard protocols for the fabrication and use of intravenous E-AB sensors.
AB - The monitoring of specific molecules in the living body has historically required sample removal (e.g., blood draws, microdialysis) followed by analysis via cumbersome, laboratory-bound processes. Those few exceptions to this rule (e.g., glucose, pyruvate, the monoamines) are monitored using “one-off” technologies reliant on the specific enzymatic or redox reactivity of their targets, and thus not generalizable to the measurement of other targets. In response we have developed in vivo electrochemical aptamer-based (E-AB) sensors, a modular, receptor-based measurement technology that is independent of the chemical reactivity of its targets, and thus has the potential to be generalizable to a wide range of analytes. To further the adoption of this in vivo molecular measurement approach by other researchers and to accelerate its ultimate translation to the clinic, we present here our standard protocols for the fabrication and use of intravenous E-AB sensors.
KW - Aptamer
KW - Electrochemical DNA biosensor
KW - In vivo sensing
KW - Square-wave voltammetry
UR - http://www.scopus.com/inward/record.url?scp=85120607415&partnerID=8YFLogxK
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U2 - 10.1007/978-1-0716-1803-5_25
DO - 10.1007/978-1-0716-1803-5_25
M3 - Chapter
C2 - 34837195
AN - SCOPUS:85120607415
T3 - Methods in Molecular Biology
SP - 479
EP - 492
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -