Electrochemical aptamer-based sensor for real-Time monitoring of insulin

Yao Wu, Beksultan Midinov, Ryan J. White

Research output: Contribution to journalArticlepeer-review


In this paper, we developed an electrochemical, aptamer-based (E-AB) for the real-Time monitoring of insulin. The sensor utilizes a redox label-modified guanine-rich aptamer which folds into a G-quadruplex for specific recognition of insulin. To develop a reproducible E-AB sensor employing insulin aptamer probes for the detection of insulin, 10% sodium dodecyl sulfate (SDS) pretreatment is crucial as it disrupts interstrand G-quartets. After sensor pretreatment with 10% SDS, a more uniform sensor response is obtained. Upon introduction of the insulin target, binding-induced steric hindrance quantitatively reduces the efficiency of electron transfer of a distal-end redox label leading to the rapid signal change within ?60 s. Testing demonstrates that the E-AB insulin exhibits a limit of detection of 20 nM and can be used to discriminate against both glucagon and somatostatin in Krebs-Ringer bicarbonate buffer, typically used in perfusion experiments. These results demonstrate that this assay has potential for rapid, specific, and quantitative analysis of insulin.

Original languageEnglish (US)
Pages (from-to)498-503
Number of pages6
JournalACS sensors
Issue number2
StatePublished - Feb 22 2019
Externally publishedYes


  • aptamers
  • electrochemistry
  • G-quadruplex
  • insulin
  • sensors

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes


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