Electroacupuncture enhances striatal neurogenesis in adult rat brains after a transient cerebral middle artery occlusion

Zeng Jin Yang, Di Han Shen, Xin Guo, Feng Yan Sun

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


In this study, we investigated the effects of electroacupuncture (EA) on ischemia -induced neurogenesis in the striatum of adult rat brains with a 30-minute middle cerebral artery occlusion. Injection of bromodeoxyuridine (BrdU, 30 mg/kg, i.p., cell proliferation marker) and 1,1′-dioctadecyl-6, 6′-di(4-sulfophenyl)-3,3′,3′,3′ -tetramethylindocarbo- cyanine (Dil, 1 μg/μl i.c.v, lipophilic neuronal tracer) combined with multiple fluorescence immunostaining were used to determine whether the proliferated cells were newly generated neurons and where they originated from in the brain. We demonstrated that EA treatment (60 Hz 1 s and 2 Hz 3 s alternately at an intensity of 10 mA for 20 min on 'Fengfu', GV.16 and "Jinsuo", GV.8) enhanced stroke-induced striatal neurogenesis in rat brains as follows: 1) EA increased the number of BrdU+ cells, indicating that it activates cell proliferation; 2) EA increased BrdU +/CRMP-4+ (collapsing response mediated protein-4, immature neuron marker) and BrdU+/MAP-2+ (microtubule-associated protein 2, mature neuron marker) cells, suggesting that it facilitates neurogenesis and maturation of newly generated neurons; 3) EA expanded the distribution of Dil-stained cells in the striatum. Moreover, most BrdU+/CRMP-4+ or BrdU+/MAP-2+ cells in the striatum were observed Dil+ staining. Thus, the results suggest that striatal newborn neurons mainly migrate from the cells lining ventricle. Therefore, we conclude that EA can improve neuronal regeneration, newborn neuron migration and their maturation in the striatum of adult rat brains after stroke.

Original languageEnglish (US)
Pages (from-to)185-199
Number of pages15
JournalAcupuncture and Electro-Therapeutics Research
Issue number3-4
StatePublished - 2005
Externally publishedYes


  • Electroacupuncture
  • Migration
  • Neurogenesis
  • Neuronal regeneration
  • Striatum
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)
  • Complementary and alternative medicine
  • Anesthesiology and Pain Medicine


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