TY - JOUR
T1 - Efferent and afferent connections of mouse sensory-motor cortex following cholinergic deafferentation at birth
AU - Hohmann, Christine F.
AU - Wilson, Lucy
AU - Coyle, Joseph T.
N1 - Funding Information:
We thank Drs. Mary Blue and James Vornov for helpful discussion of the manuscript and Alice Trawinski for editorial assistance. The present work was supported in part by U.S. Public Health Service Grant HD 19920 to J.T.C., and in part by a pilot project grant from the Alzheimer's Disease and Related Disorders Foundation to C.F.H. Correspondence should be addressed to Christine F. Hohmann, Ph.D., Department of Psychiatry, Meyer 4-163, The Johns Hopkins School of Medicine, 600 N.Wolfe Street, Baltimore, MD 21205.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1991/3
Y1 - 1991/3
N2 - The present study investigates the effect of cholinergic basal forebrain lesions at birth on cortical connectivity in adulthood. We have previously shown that such neonatal lesions result in extensive cortical cholinergic deafferentation during early postnatal development, which is accompanied by abnormal morphogenesis of cortical cytoarchitecture (Hohmann et al., 1988). Here, we have used WGA-HRP to label anterogradely and retrogradely afferent and efferent projections of dorsal neocortex. Our results show an altered projection pattern from dorsal thalamus to layer IV of sensory-motor cortex following lesions among the cholinergic basal forebrain neurons (nBM), while corticothalamic projections from layer VI appear normal. In addition, corticofugal projections from layer V, labeled by striatal injection, appear to be expanded following the lesion. This indicates that cortical layers undergoing differentiation after the newborn nBM lesion present with long-term abnormalities in connectivity. The present results are compatible with the hypothesis that cholinergic afferents are instrumental in the regulation of cortical morphogenesis. Furthermore, our data show that ontogenetic disturbances can lead to structural abnormalities that persist long after the initial deficiency has abated. We discuss the significance of these results in relationship to human neurological disorders.
AB - The present study investigates the effect of cholinergic basal forebrain lesions at birth on cortical connectivity in adulthood. We have previously shown that such neonatal lesions result in extensive cortical cholinergic deafferentation during early postnatal development, which is accompanied by abnormal morphogenesis of cortical cytoarchitecture (Hohmann et al., 1988). Here, we have used WGA-HRP to label anterogradely and retrogradely afferent and efferent projections of dorsal neocortex. Our results show an altered projection pattern from dorsal thalamus to layer IV of sensory-motor cortex following lesions among the cholinergic basal forebrain neurons (nBM), while corticothalamic projections from layer VI appear normal. In addition, corticofugal projections from layer V, labeled by striatal injection, appear to be expanded following the lesion. This indicates that cortical layers undergoing differentiation after the newborn nBM lesion present with long-term abnormalities in connectivity. The present results are compatible with the hypothesis that cholinergic afferents are instrumental in the regulation of cortical morphogenesis. Furthermore, our data show that ontogenetic disturbances can lead to structural abnormalities that persist long after the initial deficiency has abated. We discuss the significance of these results in relationship to human neurological disorders.
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U2 - 10.1093/cercor/1.2.158
DO - 10.1093/cercor/1.2.158
M3 - Article
C2 - 1726604
AN - SCOPUS:0026115274
SN - 1047-3211
VL - 1
SP - 158
EP - 172
JO - Cerebral Cortex
JF - Cerebral Cortex
IS - 2
ER -