Abstract
Background/Aim: Zeng Sheng Ping (ZSP) is a traditional herbal remedy used to prevent progression and growth of neoplastic lesions. It has been shown to inhibit Notch2 expression in a murine lung cancer model, leading us to investigate its therapeutic potential in Notch-dependent brain tumors. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)-2H-tetrazolium (MTS), apoptosis, and quantitative real-time polymerase chain reaction (RT-PCR) analyses were performed in glioma and medulloblastoma cell lines, and morphological analyses in DAOY flank xenografts. Results: ZSP inhibited brain tumor growth in vitro, in part, by apoptotic induction. Down-regulation of the Notch2 receptor, the pathway target Hairy/Enhancer of Split homolog 1 (Hes1), and of the stem cell markers Nestin and CD133 was also observed. Reductions in tumor mass and increases in the necrotic fraction of DAOY xenografts, in mice treated with oral ZSP were also observed, but these were not significant. Conclusion: ZSP can block brain tumor growth and the expression of Notch pathway members and stem cell markers in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2689-2696 |
| Number of pages | 8 |
| Journal | Anticancer research |
| Volume | 32 |
| Issue number | 7 |
| State | Published - Jul 2012 |
Keywords
- ACAPHA
- Antitumor-B
- DAOY medulloblastoma cells
- Glioblastoma
- Medulloblastoma
- Notch
- U87 glioblastoma cells
- Zeng Sheng Ping
ASJC Scopus subject areas
- Oncology
- Cancer Research