TY - JOUR
T1 - Effects of vitamin C and vitamin E on in vivo lipid peroxidation
T2 - Results of a randomized controlled trial
AU - Huang, Han Yao
AU - Appel, Lawrence J.
AU - Croft, Kevin D.
AU - Miller, Edgar R.
AU - Mori, Trevor A.
AU - Puddey, Ian B.
PY - 2002/9
Y1 - 2002/9
N2 - Background: Lipid peroxidation may be important in the pathogenesis of atherosclerosis, particularly in its earliest stages. Evidence predominantly from in vitro studies suggests that antioxidant vitamins can prevent lipid peroxidation and that vitamin C and vitamin E have synergistic effects. However, in vivo evidence in support of these hypotheses is sparse. Objective: The objective was to determine the effects of vitamin C and vitamin E, alone or in combination, on in vivo lipid peroxidation. Design: We conducted a placebo-controlled, 2 X 2 factorial trial of vitamin C (500 mg ascorbate/d) and vitamin E (400 IU RRR-α-tocopheryl acetate/d) supplementation in 184 nonsmokers. The mean duration of supplementation was 2 mo. The outcome measures were changes from baseline in urinary 8-isoprostaglandin F2α, urinary malondialdehyde + 4-hydroxyalkenals, and serum oxygen-radical absorbance capacity. Results: The within-group mean changes (and 95% CIs) in urinary 8-iso-prostaglandin F2α (pg/mg creatinine) were 9.0 (-125.1, 143.1), -150.0 (-275.4, -24.6), -141.3 (-230.5, -52.1), and -112,5 (-234.8, 9.8) in the placebo, vitamin C alone, vitamin E alone, and vitamins C + E groups, respectively. No synergistic effect of these 2 vitamins on urinary 8-iso-prostaglandin F2α was observed (P = 0.12). Neither vitamin had an effect on urinary malondialdehyde + 4-hydroxyalkenals. Vitamin C, but not vitamin E, increased serum oxygen-radical absorbance capacity (P = 0.01). Conclusions: Supplementation with vitamin C or vitamin E alone reduced lipid peroxidation to a similar extent. Supplementation with a combination of vitamins C and E conferred no benefit beyond that of either vitamin alone.
AB - Background: Lipid peroxidation may be important in the pathogenesis of atherosclerosis, particularly in its earliest stages. Evidence predominantly from in vitro studies suggests that antioxidant vitamins can prevent lipid peroxidation and that vitamin C and vitamin E have synergistic effects. However, in vivo evidence in support of these hypotheses is sparse. Objective: The objective was to determine the effects of vitamin C and vitamin E, alone or in combination, on in vivo lipid peroxidation. Design: We conducted a placebo-controlled, 2 X 2 factorial trial of vitamin C (500 mg ascorbate/d) and vitamin E (400 IU RRR-α-tocopheryl acetate/d) supplementation in 184 nonsmokers. The mean duration of supplementation was 2 mo. The outcome measures were changes from baseline in urinary 8-isoprostaglandin F2α, urinary malondialdehyde + 4-hydroxyalkenals, and serum oxygen-radical absorbance capacity. Results: The within-group mean changes (and 95% CIs) in urinary 8-iso-prostaglandin F2α (pg/mg creatinine) were 9.0 (-125.1, 143.1), -150.0 (-275.4, -24.6), -141.3 (-230.5, -52.1), and -112,5 (-234.8, 9.8) in the placebo, vitamin C alone, vitamin E alone, and vitamins C + E groups, respectively. No synergistic effect of these 2 vitamins on urinary 8-iso-prostaglandin F2α was observed (P = 0.12). Neither vitamin had an effect on urinary malondialdehyde + 4-hydroxyalkenals. Vitamin C, but not vitamin E, increased serum oxygen-radical absorbance capacity (P = 0.01). Conclusions: Supplementation with vitamin C or vitamin E alone reduced lipid peroxidation to a similar extent. Supplementation with a combination of vitamins C and E conferred no benefit beyond that of either vitamin alone.
KW - Antioxidants
KW - Free radicals
KW - Lipid peroxidation
KW - Malondialdehyde
KW - Oxygen-radical absorbance capacity
KW - Prostaglandins
KW - Vitamin C
KW - Vitamin E
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U2 - 10.1093/ajcn/76.3.549
DO - 10.1093/ajcn/76.3.549
M3 - Article
C2 - 12197998
AN - SCOPUS:0036721156
SN - 0002-9165
VL - 76
SP - 549
EP - 555
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
IS - 3
ER -