Effects of redox potential and Ca2+ on the inositol 1,4,5-trisphosphate receptor L3-1 loop region: Implications for receptor regulation

Sunmi Kang, Jinho Kang, Hyuknam Kwon, Dominique Frueh, Hyun Yoo Seung, Gerhard Wagner, Sunghyouk Park

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Inositol 1,4,5-trisphosphate receptor (IP3R) is a major intracellular Ca2+ channel, modulated by many factors in the cytosolic and lumenal compartments. Compared with cytosolic control, lumenal-side regulation has been much less studied, and some of its mechanistic aspects have been controversial. Of particular interest with regard to lumenal regulation are whether it involves direct interactions between IP3R and the regulators, and whether it involves conformational changes of the lumenal regions of IP3R. To understand these lumenal-side regulation mechanisms, we studied the effects of two important lumenal regulatory factors, the redox potential and Ca2+, on the L3-1 lumenal loop region of IP3R. The redox potential exerted direct and significant effects on the conformation of the loop region. By sharp contrast, Ca2+ showed little effect on the L3-1 conformation, suggesting that the regulation of Ca2+ is indirect or involves other receptor regions. GSH/oxidized glutathione-mediated oxidation introduced a unique intramolecular disulfide bond between Cys34 and Cys42. A variety of NMR experiments revealed that oxidation also induces localized helical characteristics in the Cys34-Cys42 region. Dynamics studies also showed reduced motions in the region upon oxidation, consistent with the conformational changes. The results raise the interesting possibility that Cys34 and Cys42 may act together as a reduction sensor, and that Cys 65 may function as an oxidation sensor. Overall, our studies suggest that the redox potential and Ca2+ can regulate IP3R through totally different mechanisms: Ca2+ by the indirect effect and the redox potential by direct action causing conformational changes.

Original languageEnglish (US)
Pages (from-to)25567-25575
Number of pages9
JournalJournal of Biological Chemistry
Issue number37
StatePublished - Sep 12 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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