Effects of prostacyclin on ovulation and microvasculature of the in vitro perfused rabbit ovary

Yasunori Yoshimura, A. M. Dharmarajan, Sanford Gips, Tomoko Adachi, Yoshihiko Hosoi, Susan J. Atlas, Edward E. Wallach

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Prostacyclin (prostaglandin I2) methyl ester at 0.1, 1, or 10 μg/ml was added to the perfusate of one rabbit ovary every 2 hours for the first 10 hours of perfusion. The contralateral ovary was perfused with medium alone. Prostacyclin methyl ester at 1 and 10 μg/ml induced ovulation in the absence of gonadotropin, with ovulatory efficiencies of 26.7% ± 3.8% and 46.5% ± 5.0%, respectively. Most ovulated ova (77.4%) did not progress beyond the germinal vesicle stage, and there was no significant degeneration of ovulated ova or follicular oocytes. Examination of the follicular microvasculature 5 hours after exposure to prostacyclin revealed marked vessel dilatation and filling defects at the apex. By 7 hours after prostacyclin exposure, the intrafollicular space contained extravasated resin, reflecting increased vascular permeability. The vascular changes paralleled those previously observed in gonadotropin-induced ovulation. These results indicate that prostacyclin acting locally alters the vascular integrity of the follicle wall and facilitates follicle rupture.

Original languageEnglish (US)
Pages (from-to)977-982
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Issue number4
StatePublished - 1988


  • Prostacyclin
  • microvasculature
  • ovarian perfusion
  • ovulation
  • rabbit

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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