TY - JOUR
T1 - Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats
AU - de Freitas, Pedro Felipe Silva
AU - Durães, Leonardo Castro
AU - de Carvalho, Felipe Augusto Neves Oliveira
AU - Duarte, Sérgio Andurte Carvalho
AU - Carneiro, Fabiana Pirani
AU - de Sousa, João Batista
PY - 2013/7
Y1 - 2013/7
N2 - Purpose: To evaluate the effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in a rat model. Methods: Twenty four rats were randomized into three groups (n=8): gasless insufflation ('open', Pressure=0 mmHg), carbon dioxide pneumoperitoneum at 12 mmHg, and helium pneumoperitoneum at 12 mmHg; all lasting 90 minutes. A cystostomy was performed and the bladder was emptied. At the end of the experiment, the urine produced, a blood sample and the left kidney of each animal were collected. The following variables were obtained: serum sodium, potassium, urea and creatinine, urine volume and creatinine. The creatinine clearance was estimated for each animal. The kidneys were stained with hematoxylin and eosin (HE) and evaluated by a pathologist blinded to the groups. Results: The CO2 and Helium groups did not differ in the variables evaluated. Both developed oliguria (p<0.001 vs. gasless). The CO2 group presented hyperkalemia compared to gasless (p=0.05), which did not attain significance in the helium group. Histopathological analysis revealed mild hydropic degeneration and congestion in the three groups, with no significant difference among them. Conclusions: The type of gas resulted in no difference in the variables of renal function and morphology assessed. The increase in serum potassium was only observed with CO2 insufflation suggests a combined effect of elevated intra-abdominal pressure and metabolic effects of pneumoperitoneum.
AB - Purpose: To evaluate the effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in a rat model. Methods: Twenty four rats were randomized into three groups (n=8): gasless insufflation ('open', Pressure=0 mmHg), carbon dioxide pneumoperitoneum at 12 mmHg, and helium pneumoperitoneum at 12 mmHg; all lasting 90 minutes. A cystostomy was performed and the bladder was emptied. At the end of the experiment, the urine produced, a blood sample and the left kidney of each animal were collected. The following variables were obtained: serum sodium, potassium, urea and creatinine, urine volume and creatinine. The creatinine clearance was estimated for each animal. The kidneys were stained with hematoxylin and eosin (HE) and evaluated by a pathologist blinded to the groups. Results: The CO2 and Helium groups did not differ in the variables evaluated. Both developed oliguria (p<0.001 vs. gasless). The CO2 group presented hyperkalemia compared to gasless (p=0.05), which did not attain significance in the helium group. Histopathological analysis revealed mild hydropic degeneration and congestion in the three groups, with no significant difference among them. Conclusions: The type of gas resulted in no difference in the variables of renal function and morphology assessed. The increase in serum potassium was only observed with CO2 insufflation suggests a combined effect of elevated intra-abdominal pressure and metabolic effects of pneumoperitoneum.
KW - Artificial
KW - Carbon Dioxide
KW - Helium
KW - Kidney
KW - Laparoscopy
KW - Pneumoperitoneum
KW - Rats
KW - Surgery
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U2 - 10.1590/S0102-86502013000700003
DO - 10.1590/S0102-86502013000700003
M3 - Article
C2 - 23842929
AN - SCOPUS:84880392908
SN - 0102-8650
VL - 28
SP - 494
EP - 498
JO - Acta cirurgica brasileira
JF - Acta cirurgica brasileira
IS - 7
ER -