Abstract
The effects of multiple freeze-thaw cycles and various temperatures (-20°C, 4°C, 25°C, 37°C) on the reactivity of human immunodeficiency virus (HIV) antibodies were evaluated using current ELISA, recombinant, and Western blot methodologies. Twenty consecutive freeze-thaw cycles and storage of specimens at -20°C and 4°C for 57 days resulted in no loss of HIV antibody reactivity nor false positive samples. Maintenance of clinical specimens at 25°C and 37°C for 57 days resulted in some loss of HIV antibody reactivity, but all positive and weakly reacting samples remained positive, and negative samples were unaffected.
Original language | English (US) |
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Pages (from-to) | 127-132 |
Number of pages | 6 |
Journal | Journal of Virological Methods |
Volume | 20 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1988 |
Keywords
- ELISA
- Freeze-thaw cycles
- HIV antibody reactivity
- Recombinant
- Specimens, positive and negative
- Western blot
ASJC Scopus subject areas
- Virology