Abstract
2 Deoxyglucose and glucosamine were found to inhibit cell fusion caused by a syncytial mutant of herpes simplex virus and to inhibit the glycosylation of viral glycoproteins in the infected cells. The inhibition of fusion and the inhibition of glycosylation caused by 2 deoxyglucose were substantially prevented when mannose was also present during infection. When glycosylation was inhibited, 3 new bands were found in the major glycoprotein region on sodium dodecyl sulfate polyacrylamide gels. These bands may be precursors to the normal glycoproteins. The correlation between fusion and glycosylation in the presence of 2 deoxyglucose, glucosamine, and mannose suggests that the cells cannot fuse if their glycoproteins have a considerably reduced carbohydrate content.
Original language | English (US) |
---|---|
Pages (from-to) | 644-651 |
Number of pages | 8 |
Journal | Journal of virology |
Volume | 18 |
Issue number | 2 |
DOIs | |
State | Published - 1976 |
Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology