Effective removal of albumin from serum

David A. Colantonio; Christy Dunkinson; Diane E. Bovenkamp; Jennifer E. Van Eyk

doi:10.1002/pmic.200401235

Effective removal of albumin from serum

David A. Colantonio, Christy Dunkinson, Diane E. Bovenkamp, Jennifer E. Van Eyk

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.

Original language	English (US)
Pages (from-to)	3831-3835
Number of pages	5
Journal	Proteomics
Volume	5
Issue number	15
DOIs	https://doi.org/10.1002/pmic.200401235
State	Published - Oct 2005
Externally published	Yes

Keywords

2-DE
Albumin
Enrichment
Serum proteome

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1002/pmic.200401235

Cite this

@article{60d53f8eef6b4a5183ccdaec5bc16315,

title = "Effective removal of albumin from serum",

abstract = "The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.",

keywords = "2-DE, Albumin, Enrichment, Serum proteome",

author = "Colantonio, {David A.} and Christy Dunkinson and Bovenkamp, {Diane E.} and {Van Eyk}, {Jennifer E.}",

year = "2005",

month = oct,

doi = "10.1002/pmic.200401235",

language = "English (US)",

volume = "5",

pages = "3831--3835",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "15",

}

TY - JOUR

T1 - Effective removal of albumin from serum

AU - Colantonio, David A.

AU - Dunkinson, Christy

AU - Bovenkamp, Diane E.

AU - Van Eyk, Jennifer E.

PY - 2005/10

Y1 - 2005/10

N2 - The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.

AB - The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.

KW - 2-DE

KW - Albumin

KW - Enrichment

KW - Serum proteome

UR - http://www.scopus.com/inward/record.url?scp=27144448180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27144448180&partnerID=8YFLogxK

U2 - 10.1002/pmic.200401235

DO - 10.1002/pmic.200401235

M3 - Article

C2 - 16145708

AN - SCOPUS:27144448180

SN - 1615-9853

VL - 5

SP - 3831

EP - 3835

JO - Proteomics

JF - Proteomics

IS - 15

ER -

Effective removal of albumin from serum

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this