Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique

David E. Wolf; Alan H. Handyside; Michael Edidin

doi:10.1016/S0006-3495(82)84561-X

Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique

David E. Wolf
, Alan H. Handyside
, Michael Edidin

School of Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10^-9 cm²/s with (77.0 ± 2.1)% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10^-9 cm²/s with (84.7 ± 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.

Original language	English (US)
Pages (from-to)	295-297
Number of pages	3
Journal	Biophysical journal
Volume	38
Issue number	3
DOIs	https://doi.org/10.1016/S0006-3495(82)84561-X
State	Published - 1982

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(82)84561-X

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@article{28ce4bff57d6453885a1879897d8abb8,

title = "Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique",

abstract = "To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10-9 cm2/s with (77.0 ± 2.1)\% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10-9 cm2/s with (84.7 ± 1.3)\% recovery. We thus find only slight differences in diffusion in the two regions.",

author = "Wolf, \{David E.\} and Handyside, \{Alan H.\} and Michael Edidin",

note = "Funding Information: We wish to acknowledge Ms. Linda Hall for her help in the preparation of this manuscript and to thank Drs. B. Aizenbud and N. Gershon for their interest in this problem. This work was supported in part by National Institutes of Health (NIH) grant AI-14584 to Dr. Edidin; an NIH postdoctoral fellowship CA-06399 to Dr. Wolf; and the Wellcome Trust to Dr. Handyside. This is a contribution No. 1139 of The Johns Hopkins University, Department of Biology.",

year = "1982",

doi = "10.1016/S0006-3495(82)84561-X",

language = "English (US)",

volume = "38",

pages = "295--297",

journal = "Biophysical journal",

issn = "0006-3495",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique

AU - Wolf, David E.

AU - Handyside, Alan H.

AU - Edidin, Michael

N1 - Funding Information: We wish to acknowledge Ms. Linda Hall for her help in the preparation of this manuscript and to thank Drs. B. Aizenbud and N. Gershon for their interest in this problem. This work was supported in part by National Institutes of Health (NIH) grant AI-14584 to Dr. Edidin; an NIH postdoctoral fellowship CA-06399 to Dr. Wolf; and the Wellcome Trust to Dr. Handyside. This is a contribution No. 1139 of The Johns Hopkins University, Department of Biology.

PY - 1982

Y1 - 1982

N2 - To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10-9 cm2/s with (77.0 ± 2.1)% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10-9 cm2/s with (84.7 ± 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.

AB - To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10-9 cm2/s with (77.0 ± 2.1)% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10-9 cm2/s with (84.7 ± 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.

UR - https://www.scopus.com/pages/publications/0020149797

UR - https://www.scopus.com/pages/publications/0020149797#tab=citedBy

U2 - 10.1016/S0006-3495(82)84561-X

DO - 10.1016/S0006-3495(82)84561-X

M3 - Article

C2 - 7104441

AN - SCOPUS:0020149797

SN - 0006-3495

VL - 38

SP - 295

EP - 297

JO - Biophysical journal

JF - Biophysical journal

IS - 3

ER -

Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique

Abstract

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