Effect of human hydrosalpinx fluid on murine embryo development and implantation

Objective: To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation. Design: The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined. Setting: Private hospital-based fertility center and IVF program. Main Outcome Measure(s): Percentage of embryos continuing cell division and implantation rates after ET. Result(s): Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation. Conclusion(s): Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.