@article{9ce4724eeecd4adabbea77aa69ec3dd6,
title = "Effect of enterotoxins of Vibrio cholerae, Escherichia coli, and Shigella dysenteriae type 1 on fluid and electrolyte transport in the colon",
abstract = "Experiments were designed to determine whether the enterotoxins of Vibrio cholerae, E. coli, and Shigella dysenteriae type 1 alter the movement of fluid and electrolytes in the rat cecum. Net secretion of water as sodium were observed after incubation of 1.67 x 10-5 μg of purified cholera toxin (choleragen)/ml for 18 hr or of 50 μg/ml for 3 hr. The effect of choleragen on cecal transport of water and electrolytes was related to the dose. In addition, choleragen increased cecal mucosal content of adenosine 3':5' cyclic phosphate but did not alter the histology of the cecum. The results demonstrate that the colon responds to choleragen in a manner similar to that of other tissues. In contrast, the enterotoxins of both E. coli and S. dysenteriae type 1 failed to affect cecal transport of water and electrolytes. These observations may explain several phenomena associated with the diarrhea produced by bacterial enterotoxins.",
author = "M. Donowitz and Binder, {H. J.}",
note = "Funding Information: Received for publication October 16, 1975, and in revised form February 9, 1976. This work was supported by grant no. AM 14669 from the National Institute of Arthritis, Metabolism, and Digestive Diseases, and by grants from the John A. Hartford Foundation, Inc., and the Connecticut Digestive Disease Society. Dr. Donowitz was supported by training grant no. AM 05703 from the National Institute of Arthritis, Metabolism, and Digestive Diseases. We acknowledge the expert technical assistance of J. M. Heffernan, P. Fisher, D. Whiting, and G.Chapo, and the secretarial assistance of Carol Bryan and Elizabeth Penn. We thank Drs. Carl E. Miller (National Institute of Allergy and Infectious Diseases), Thomas M. Jacks (Norwich Pharmacal Company, Norwich, N. Y.), Samuel Formal (Walter Reed Army Institute of Research), and Gerald T. Keusch (Mt. Sinai School of Medicine, New York, N.Y.) for their kind gifts of enterotoxins. In conducting the research described in this paper, the investigators adhered to the {"}Guide for Laboratory Animal Facilities and Care,{"} as promulgated by the Committee on the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources, National Academy of Sciences-National Research Council. Please address request for reprints to Dr. Mark Donowitz, Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D.C. 20012.",
year = "1976",
doi = "10.1093/infdis/134.2.135",
language = "English (US)",
volume = "134",
pages = "135--143",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",
}