TY - JOUR
T1 - Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas
AU - Longnecker, Daniel S.
AU - French, Janice
AU - Hyde, Elisabeth
AU - Lilja, Herman S.
AU - Yager, James D.
N1 - Funding Information:
ABBREVIATIONS USED: TdR = thymidine; H & E = hematoxylin and eosin; TCA = trichloroacetic acid. 1 Received August 30, 1976; accepted December 21, 1976. 2 Supported by Public Health Service contracts NOI-CP33378 from the Division of Cancer Cause and Prevention and NOI-CN55199 from the Division of Cancer Control, and by Public Health Service grant CA17843 from the National Cancer Institute (NCI). 3 A portion of the azaserine used in these studies was supplied by the Drug Development Branch (Dr. H. B. Wood, Jr.), Division of Cancer Treatment, NCI. 4 Department of Pathology, Dartmouth Medical School, Hanover, N.H. 03755. 5 We thank Maureen Devine and Richard Markley for technical assistance.
PY - 1977/6
Y1 - 1977/6
N2 - The efficacy of various azaserine treatment durations was evaluated with respect to induction of atypical acinar cell nodules in Wistar rat pancreas and was related to animal age and rate of pancreatic DNA synthesis during growth. The sensitivity to nodule induction was maximal in postnatal rats when the rate of pancreatic DNA synthesis was high, whereas treatment of weanlings was less effective and treatment of mature rats was least effective. When weaned growing rats were given 1, 3, or 5 weekly injections of 30 mg azaserine/kg, the number of nodules induced was proportional to the number of injections. A single dose at this level did not induce detectable pancreatic necrosis or inflammation; therefore, DNA synthesis due to regeneration was probably not a major factor in the initiation of nodules. We concluded that multiple daily injections of [3H]thymidine during the first or second postnatal week provided DNA of sufficiently high specific activity for use in DNA repair and biochemical toxicity studies.
AB - The efficacy of various azaserine treatment durations was evaluated with respect to induction of atypical acinar cell nodules in Wistar rat pancreas and was related to animal age and rate of pancreatic DNA synthesis during growth. The sensitivity to nodule induction was maximal in postnatal rats when the rate of pancreatic DNA synthesis was high, whereas treatment of weanlings was less effective and treatment of mature rats was least effective. When weaned growing rats were given 1, 3, or 5 weekly injections of 30 mg azaserine/kg, the number of nodules induced was proportional to the number of injections. A single dose at this level did not induce detectable pancreatic necrosis or inflammation; therefore, DNA synthesis due to regeneration was probably not a major factor in the initiation of nodules. We concluded that multiple daily injections of [3H]thymidine during the first or second postnatal week provided DNA of sufficiently high specific activity for use in DNA repair and biochemical toxicity studies.
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U2 - 10.1093/jnci/58.6.1769
DO - 10.1093/jnci/58.6.1769
M3 - Article
C2 - 864754
AN - SCOPUS:0017664680
SN - 0027-8874
VL - 58
SP - 1769
EP - 1775
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -