Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone

Francois Godeau; Teruko Ishizaka; S. S. Koide

doi:10.1016/0045-6039(85)90605-0

Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone

Francois Godeau, Teruko Ishizaka, S. S. Koide

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Progesterone induced a transient increase in the incorporation of [³H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in ⁴⁵Ca²⁺ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Cell Differentiation
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0045-6039(85)90605-0
State	Published - Feb 1985
Externally published	Yes

Keywords

Xenopus
oocyte maturation
phospholipid-methylation
progesterone

ASJC Scopus subject areas

Developmental Biology

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10.1016/0045-6039(85)90605-0

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@article{87cc607464da497eb4caecb688bc16fa,

title = "Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone",

abstract = "Progesterone induced a transient increase in the incorporation of [3H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in 45Ca2+ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.",

keywords = "Xenopus, oocyte maturation, phospholipid-methylation, progesterone",

author = "Francois Godeau and Teruko Ishizaka and Koide, {S. S.}",

note = "Funding Information: This study was supported in part by grant No, ROI-HD-13184 from the NICHHD, All0060 from NIAID and GA PS 8310 from the Rockefeller Foundation. The authors express their appreciation to Mr. Alan R. Sterk for his excellent technical assistance. This article is publication No. 586 from the O'Neill Laboratories at the Good Samaritan Hospital.",

year = "1985",

month = feb,

doi = "10.1016/0045-6039(85)90605-0",

language = "English (US)",

volume = "16",

pages = "35--41",

journal = "Cell Differentiation",

issn = "0045-6039",

publisher = "John Wiley & Sons Inc.",

number = "1",

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TY - JOUR

T1 - Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone

AU - Godeau, Francois

AU - Ishizaka, Teruko

AU - Koide, S. S.

N1 - Funding Information: This study was supported in part by grant No, ROI-HD-13184 from the NICHHD, All0060 from NIAID and GA PS 8310 from the Rockefeller Foundation. The authors express their appreciation to Mr. Alan R. Sterk for his excellent technical assistance. This article is publication No. 586 from the O'Neill Laboratories at the Good Samaritan Hospital.

PY - 1985/2

Y1 - 1985/2

N2 - Progesterone induced a transient increase in the incorporation of [3H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in 45Ca2+ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.

AB - Progesterone induced a transient increase in the incorporation of [3H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in 45Ca2+ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.

KW - Xenopus

KW - oocyte maturation

KW - phospholipid-methylation

KW - progesterone

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AN - SCOPUS:0021907998

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JO - Cell Differentiation

JF - Cell Differentiation

IS - 1

ER -

Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone

Abstract

Keywords

ASJC Scopus subject areas

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