E-rosette receptors induced by phytohemagglutinin on human K cells expressing T-cell surface antigens

Edwin W. Ades; Patricia Dougherty; Steven L. Shore; Charles M. Balch

doi:10.1016/0008-8749(79)90038-8

E-rosette receptors induced by phytohemagglutinin on human K cells expressing T-cell surface antigens

Edwin W. Ades, Patricia Dougherty, Steven L. Shore, Charles M. Balch

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Killer cells (K cells) enriched from human blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) were examined for surface markers. Sixty-seven percent of the E-rosette-negative, sIg-negative cells reacted with anti-T cell serum (AMT) previously shown to react with immunochemically defined T-cell antigens. Phytohemagglutinin induced 25% of K cells to express an E-rosette receptor. When these induced cells were isolated, greater than 98% reacted with AMT and 17% expressed the Fc receptor for IgG. Furthermore, they retained their functional capacity in ADCC. These findings demonstrate that an E-rosette receptor can be induced on human K cells. The data suggest the K-cell fraction included a population of thymus-dependent lymphocytes which can function as effector cells in ADCC.

Original language	English (US)
Pages (from-to)	179-185
Number of pages	7
Journal	Cellular Immunology
Volume	44
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(79)90038-8
State	Published - Apr 1979
Externally published	Yes

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(79)90038-8

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title = "E-rosette receptors induced by phytohemagglutinin on human K cells expressing T-cell surface antigens",

abstract = "Killer cells (K cells) enriched from human blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) were examined for surface markers. Sixty-seven percent of the E-rosette-negative, sIg-negative cells reacted with anti-T cell serum (AMT) previously shown to react with immunochemically defined T-cell antigens. Phytohemagglutinin induced 25% of K cells to express an E-rosette receptor. When these induced cells were isolated, greater than 98% reacted with AMT and 17% expressed the Fc receptor for IgG. Furthermore, they retained their functional capacity in ADCC. These findings demonstrate that an E-rosette receptor can be induced on human K cells. The data suggest the K-cell fraction included a population of thymus-dependent lymphocytes which can function as effector cells in ADCC.",

author = "Ades, {Edwin W.} and Patricia Dougherty and Shore, {Steven L.} and Balch, {Charles M.}",

note = "Funding Information: r This investigation was supported by grants from the Medical Research Service of the Veteran{\textquoteright}s Administration and from the National Institute of Health (CA13148 CA16673). 2 Recipient of a Special Fellowship from the Leukemia Society of America. 3 Recipient of a Faculty Fellowship in Oncology from the American Cancer Society. 4 To whom requests for reprints should be sent: Department of Surgery, 750 Lyons-Harrison Research, University of Alabama Medical Center, Birmingham, Ala. 35294. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1979",

month = apr,

doi = "10.1016/0008-8749(79)90038-8",

language = "English (US)",

volume = "44",

pages = "179--185",

journal = "Cellular Immunology",

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publisher = "Academic Press Inc.",

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T1 - E-rosette receptors induced by phytohemagglutinin on human K cells expressing T-cell surface antigens

AU - Ades, Edwin W.

AU - Dougherty, Patricia

AU - Shore, Steven L.

AU - Balch, Charles M.

N1 - Funding Information: r This investigation was supported by grants from the Medical Research Service of the Veteran’s Administration and from the National Institute of Health (CA13148 CA16673). 2 Recipient of a Special Fellowship from the Leukemia Society of America. 3 Recipient of a Faculty Fellowship in Oncology from the American Cancer Society. 4 To whom requests for reprints should be sent: Department of Surgery, 750 Lyons-Harrison Research, University of Alabama Medical Center, Birmingham, Ala. 35294. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1979/4

Y1 - 1979/4

N2 - Killer cells (K cells) enriched from human blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) were examined for surface markers. Sixty-seven percent of the E-rosette-negative, sIg-negative cells reacted with anti-T cell serum (AMT) previously shown to react with immunochemically defined T-cell antigens. Phytohemagglutinin induced 25% of K cells to express an E-rosette receptor. When these induced cells were isolated, greater than 98% reacted with AMT and 17% expressed the Fc receptor for IgG. Furthermore, they retained their functional capacity in ADCC. These findings demonstrate that an E-rosette receptor can be induced on human K cells. The data suggest the K-cell fraction included a population of thymus-dependent lymphocytes which can function as effector cells in ADCC.

AB - Killer cells (K cells) enriched from human blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) were examined for surface markers. Sixty-seven percent of the E-rosette-negative, sIg-negative cells reacted with anti-T cell serum (AMT) previously shown to react with immunochemically defined T-cell antigens. Phytohemagglutinin induced 25% of K cells to express an E-rosette receptor. When these induced cells were isolated, greater than 98% reacted with AMT and 17% expressed the Fc receptor for IgG. Furthermore, they retained their functional capacity in ADCC. These findings demonstrate that an E-rosette receptor can be induced on human K cells. The data suggest the K-cell fraction included a population of thymus-dependent lymphocytes which can function as effector cells in ADCC.

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E-rosette receptors induced by phytohemagglutinin on human K cells expressing T-cell surface antigens

Abstract

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