Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging

Min Ho Kim, Wei Liu, Dori L. Borjesson, Fitz Roy E. Curry, Lloyd S. Miller, Ambrose L. Cheung, Fu Tong Liu, R. Rivkah Isseroff, Scott I. Simon

Research output: Contribution to journalArticlepeer-review

141 Scopus citations


Neutrophil influx is an early inflammatory response that is essential for the clearance of bacteria and cellular debris during cutaneous wounding. A non-invasive real-time fluorescence imaging technique was developed to examine the kinetics of enhanced green fluorescence protein-polymorphonuclear leukocyte (EGFP-PMN) influx within a wound. We hypothesized that infection or systemic availability would directly regulate the dynamics of EGFP-PMN recruitment and the efficiency of wound closure. Neutrophil recruitment increased dramatically over the first 24 hours from 106 at 4 hours up to a maximum of 5 × 106 EGFP-PMNs at 18 hours. A high rate of EGFP-PMN turnover was evidenced by ∼80% decrease in EGFP signal within 6 hours. In response to wound colonization by Staphylococcus aureus or injection of GM-CSF, systemic PMNs increased twofold above saline control. This correlated with an increase in EGFP-PMN recruitment up to ∼107 within the wound. Despite this effect by these distinct inflammatory drivers, wound closure occurred at a rate similar to the saline-treated control group. In summary, a non-invasive fluorescence-based imaging approach combined with genetic labeling of neutrophils provides a dynamic inner view of inflammation and the kinetics of neutrophil infiltration into the wounded skin over extended durations.

Original languageEnglish (US)
Pages (from-to)1812-1820
Number of pages9
JournalJournal of Investigative Dermatology
Issue number7
StatePublished - Jul 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology


Dive into the research topics of 'Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging'. Together they form a unique fingerprint.

Cite this