Abstract
The neural crest (NC) is a transient population of multipotent cells giving rise to the peripheral nervous system, skin pigmentation, heart, and facial mesenchyme. The broad cell fate potential of NC makes it an attractive cell fate to derive from human pluripotent stem cells (hPSCs) for exploring embryonic deve lopment,modeling disease, and generating cells for transplantation. Here, we discuss recent publications and methods for efficiently differentiating hPSCs into NC. We also provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons.
Original language | English (US) |
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Pages (from-to) | 329-343 |
Number of pages | 15 |
Journal | Methods in Molecular Biology |
Volume | 1307 |
DOIs | |
State | Published - 2015 |
Keywords
- Disease modeling
- Dual SMAD inhibition
- Human pluripotent stem cells
- Melanocyte
- Neural crest
- Peripheral sensory neurons
ASJC Scopus subject areas
- Molecular Biology
- Genetics